After burn injury, complicated altered cytokine profiles are caused by toll-like receptor 2 and 4 ligation. Toll-like receptor TLR2 and TLR4, expressed on innate immune cells, are important mediators of the immune response to pathogens.
Background:
In this study, we hypothesized that burn injury would lead to altered cytokine secretion profiles following her TLR2 or TLR4 ligation, associated with changes in her TLR expression in innate immune cells.
METHODS:
Female C56BL/6 mice were subjected to full-thickness burns or sham wounds of her 20%. At 3 or 14 days after her injury, whole spleen cells or purified splenic macrophages were cultured with her TLR2 ligand peptidoglycan or her TLR4 ligand lipopolysaccharide.
Supernatants were tested for TNF-α, MCP-1, IL-6, and IL-10. Cell death was assessed using flow cytometry. CD11b F4/80 congenital macrophages were sorted 14 days after burn injury and TLR2 and 4 expression was determined by quantitative reverse transcriptase polymerase chain reaction and flow cytometry.
RESULTS:
Burn injury results in a stable accumulation of CD11bF4/80 macrophages in the periphery. Purified macrophages early after burn injury upregulated TLR2 and 4, followed by a decrease in TLR2 and TLR4 expression late after burn injury.
Equivalent numbers of TLR2 and TLR4 ligated purified macrophages 3 days after thermal injury revealed no significant difference in cytokine secretion compared to sham. Stimulation 14 days after burn injury showed a significant decrease in tumor necrosis factor-α secretion by macrophages compared to sham mice.
In contrast, in the late post-burn period, interleukin-10 was significantly increased (mean approximately 1.8-fold) after either TLR2 or TLR4 stimulation. Interleukin-6 and monocyte chemoattractant protein-1 secretion were unchanged from sham levels.
In contrast, stimulation of whole splenocytes increased cytokines 3 and 14 days after burn injury. This effect may be caused by the accumulation of His TLR macrophages, which are resistant to TLR-induced cell death.
CONCLUSIONS:
Cytokine secretion profiles after TLR2 and TLR4 ligation after burn injury are altered in ways that do not clearly reflect anti- or pro-inflammatory states and are associated with intrinsic changes in populations. TLR2 and TLR4 ligation play complex and diverse roles in mediating the immune response to burn injury.
To learn more Macrophages click below-
https://brainly.com/question/28496020
#SPJ4
In our solar system alone, eight known planets orbit around our sun. knowing this, approximately how many planets orbit other stars within the milky way galaxy?
3,800 planets orbit other stars within the Milky Way galaxy.
Astronomers have verified the presence of roughly 3,800 planets circling stars other than the Sun as of July 2018. The eight planets of our Solar System; Mercury, Venus, Earth, Mars, Jupiter, Saturn, Uranus, and Neptune, are the most well-known planets in our Milky Way. Pluto, Eris, Makemake, Haumea, and Ceres are also dwarf planets. Exoplanets are planets that are not in our Solar System but are situated in the Milky Way.
Our solar system is only one type of planetary system—a star with planets around it. Although our planetary system is the only one officially designated as a "solar system," scientists have identified more than 3,200 additional stars in our galaxy with planets around them. Hence, 3800 planets have been discovered so far.
To learn more about solar system. Click, https://brainly.com/question/18365761
#SPJ4
By reviewing hiring information from the previous year, james is trying to predict how many additional employees he will need to hire for the holiday rush. Which step in the process of human resource planning is jim engaged in?.
By reviewing hiring information from the previous year, James is trying to predict how many additional employees he will need to hire for the holiday rush. James is engaged in the step of a. forecasting in the process of human resource planning.
It is through proper human resource planning that a company recognizes the number and quality of team members that are required for the better performance of a company.
A human resource employee, such as James, first takes into account the current capacity of hiring. Then he reviews previous hiring information of the holiday season to forecast how many employees need to be hired.
The forecast process helps the company to have the proper amount of workers needed during the holiday rush. After forecasting the required number of hires to be made, one can form an action plan and evaluate people for the hiring process.
Although a part of your question is missing, you might be referring to this question:
By reviewing hiring information from the previous year, James is trying to predict how many additional employees he will need to hire for the holiday rush. Which step in the process of human resource planning is James engaged in?
a. forecasting
b. evaluation
c. talent strategy
To learn more about human resource planning, click here:
https://brainly.com/question/13761208
#SPJ4
Wheat produces starch and cellulose, both of which are polysaccharides. While we rely on one for energy, we cannot digest the other. What causes such a physiological difference in digestion?.
Our bodies have the capacity to break the bonds that hold the various glucose molecules together.
Where do glucose molecules reside?Glucose is a member of the carbohydrate family. It is the most significant source of energy for all living things on Earth and is a monosaccharide (simple sugar). It is abundantly present in plants, fruit (especially berries), and honey.
What role does a glucose molecule have in the body?The glucose molecule's main function is to serve as a fuel or source of energy. Glucose is used by both plants and animals as a liquid, widely dispersed source of chemical energy that may be "burnt" in the mitochondria and cytoplasm to produce carbon dioxide, water, and energy.
To know more about Glucose molecule visit:
https://brainly.com/question/28295470
#SPJ4
why is it easier/ faster for our body to digest candy than pasta? candy contains disaccharides and thus contains more substrate and needs more enzyme to digest. pasta contains glycogen and thus contains more substrate and needs more enzyme to digest. candy contains monosaccharides and thus contains more substrate and needs more enzyme to digest. pasta contains starch and thus contains more substrate and needs more enzyme to digest.
Pasta contains starch and thus contains more substrate and needs more enzyme to digest.
What is starch ?A polymeric carbohydrate called starch, also known as amylum, is made up of a lot of glucose units connected by glycosidic linkages. The majority of green plants synthesize this polysaccharide as a form of energy storage. It is the most prevalent type of carbohydrate consumed by people worldwide and is present in significant proportions in common foods like wheat, potatoes, maize (corn), rice, and cassava (manioc).
Pure starch is a powder that is white, odorless, tasteless, and insoluble in alcohol or cold water. It is made up of the branching amylopectin and the linear and helical amylose molecules. Starch typically comprises 20 to 25% amylose and 75 to 80% amylopectin by weight, depending on the plant. Animals store their energy in glycogen, which is a more intricately branched form of amylopectin.
To learn more about starch from the given link:
https://brainly.com/question/1237142
#SPJ4
Embryology is the study of the development of an , which is a fertilized egg attached to the wall of the mother's uterus.
I know I copied and pasted this but I’m time on a unit test!! Please help!
Answer: true
Explanation:
How do the sporophyte stage and gametophyte stage make a cycle ?
The diploid zygote, which eventually becomes the sporophyte, is produced by the fusion of the male and female gametes. When a diploid sporophyte reaches maturity, it produces spores by meiosis, which then divide through mitosis to create the haploid gametophyte. The cycle is continued when a fresh gametophyte develops gametes.
What is Sporophyte and gametophyte stage ?The sporophyte symbolises the asexual phase of the plant life cycle, and the gametophyte the sexual phase. Gametophyte and sporophyte are fundamentally different from one another because gametophyte is a haploid and created through meiosis, whilst sporophyte is a diploid and formed through mitosis.
Ferns go through two distinct stages in their life cycle: sporophyte, which releases spores, and gametophyte, which releases gametes. Sporophyte plants are diploid, whereas gametophyte plants are haploid. Alternation of generations is the name given to this type of life cycle.Learn more about Plant life cycle here:
https://brainly.com/question/22729923
#SPJ9
give 2-3 examples of fats.
Answer: Trans fat Saturated Fat And Unsaturated Fat
Explanation:
Answer:
Lipids
Fatty acids
Oils
i hope this helps you
which sentence is an example of how a scientist might use a model?a microscope is used to magnify a group of cells on a slide.a microscope is used to magnify a group of cells on a slide.the end of a slinky is moved vertically up and down to simulate a wave.the end of a slinky is moved vertically up and down to simulate a wave.a wrench is used to tighten a nut onto a piece of wood.a wrench is used to tighten a nut onto a piece of wood.a stopwatch is used to time the rate of a chemical reaction.a stopwatch is used to time the rate of a chemical reaction.
Correct answer is B. The proper response is choice To create the illusion of a wave, the end of a slinky is moved vertically up and down to simulate a wave.
A scientific model is a visual representation of concepts or a reality that cannot be directly experienced. One cannot actually perceive the waves' motion. So, to illustrate the nature of waves, a scientist or somebody uses a slinky that is vertically raised and lowered.
What is the motion of waves?A disturbance that moves across the medium is called a wave motion. Simple harmonic motion is executed by the wave's particles around the mean location as it moves through the medium. Both kinetic and potential energy will be present in the medium's vibrating particles.
To learn more about science model from given link
https://brainly.com/question/4702227
#SPJ4
Which of the combination of atoms or elements below represents Nucleic Acid.
A. C H O
B. C H O N
C. C H O N P
D. None of the above
Help this assignment has to be done by today and what does this even go under?
If an individual were unable to produce sebum, what problem would he or she most likely develop?
If an individual were unable to produce sebum, the problem that he or she would most likely develop is he or she would have brittle, more easily broken hair and is denoted as option A.
What is Sebum?This is referred to an oily and waxy substance which is produced by the sebaceous gland in the skin and are responsible for ensuring that the skin is very hydrated.
This substance is made up of mostly lipids and it also ensures that the skin has a protective coating thereby protecting it from foreign bodies such as pathogens , dust etc.
The inability of an individual to produce it means that the hair being brittle and easily broken will result in this type of condition. This is therefore the reason why option A was chosen as the most appropriate choice.
Read more about Sebaceous gland here https://brainly.com/question/10649665
#SPJ1
The options are:
A. He or she would have brittle, more easily broken hair.
B. He or she would be unable to make keratin.
C. He or she would have excessively oily skin.
D. He or she would be less likely to get sunburns.
You start with 100 units of protein activity and 100 grams of total protein. After the first centrifugation step, you have 15% less activity and 80% less total protein. What is your fold-purification of the target protein?.
We have 4.25 fold-purification of target protein.
Protein purification is a set of procedures designed to separate one or more proteins from a complicated mixture, often cells, organs, or whole organisms. Protein purification is essential for determining the function, structure, and relationships of a protein of interest. The number of times a protein preparation is enriched for the protein being purified is referred to as fold purification. If a protein concentration is 2nM before purification and 20nM after purification, the fold purification is twenty-fold.
Initial protein activity = 100 units
% change in protein activity = 15%
Final protein activity = 85%
Initial total protein = 100 grams
% change in total protein =80%
Final total protein = 20%
Fold purification = final protein activity / total protein
=85/20
= 4.25
Therefore, we have 4.25 fold-purification of target protein
To learn more about the steps of purification. Click, https://brainly.com/question/3130146
#SPJ4
Biologists sometimes use different models or systems to represent things they cannot study directly. True or false?.
True , biologist sometimes use different models to represent they cannot study directly.
What are scientific models explain why scientists create and use scientific models?A scientific model is a physical and/or mathematical and/or conceptual representation of a system of ideas, events or processes. Scientists seek to identify and understand patterns in our world by drawing on their scientific knowledge to offer explanations that enable the patterns to be predicted.
Models help us to understand the concepts for easily in 3D form. Scientist use models to investigate objects or processes that happen too slowly, too quickly.
To learn more about Models refer
https://brainly.com/question/1428184
#SPJ4
four characteristic that exchange surfaces have in common
If zebras and horses do not usually mate in nature, should people intentionally cross them? Why or why not?
Answer: yes they often do intentionally sometimes
Explanation:
to produce a new breed with new characters.
ANATOMY & PHYSIOLOGY 40 PTS EACH
the small intestine is a digestive organ that is responsible for absorption of nutrients during the digestion process. It contains tissue that is only one cell thick, and readily divides . These also tend to have a larger surface area. these qualities make it easy for substances to be absorbed.
Based on the passage above, which type of tissue will be found lining the small intestine?
-Muscle
-Epithelial
-connective
-nervous
The type of the tissue found in the lining of the small intestine will be epithelial tissue.
Small intestine is the organ of the digestive system. It is a long tube like structure that is fitted to a a small space all intertwined in itself. The function of the small intestine is to perform the digestion of foods and also aids in its absorption.
Epithelial tissue is the one present on the outer layer of the skin and in the internal lining of the organs. It is made up of a single layer of cells. This is to mediate the transport and secretion of substances from their membrane.
To know more about epithelial tissue, here
brainly.com/question/13404204
#SPJ1
Answer: Epithelial
Explanation:
All of the following influence tides except A. The sun B. The moon C. The air temperature and D. The rotation of the earth
Answer:
C . the air temperature
Explanation:
i know for a fact that the moon does . the sun affects it twice as much as the moon . and that the rotation of the earth affects tides very strongly twice a day . and i couldnt find anything for air temperature so thats gotta be it . hope this helps .
Why do you think the bones of the lower limbs are thicker than the bones of the upper limbs?
Answer:
Because the bones of the lower limbs have to be able to support the entire weight of the body
Explanation:
Hope this helps :)
Which of the following best predicts what biome will be present in an area?
A biome is a sizable region distinguished by its flora, fauna, soil, and climate. Temperature and precipitation will be present in biome in an area.
What is biome?A biome is a sizable region distinguished by its flora, fauna, soil, and climate.
Aquatic, grassland, forest, desert, and tundra biomes are the five main types, while some of these can be further broken down into more specialized groups.
Due to various elements, such as water and temperature change, which prevent other biomes from being more stable, the marine biome is the most stable. Since oceans don't have "seasons," there is always a supply of water, and temperature changes are often small.
Precipitation and temperature predicts the biome will be present in an area.
Therefore, A biome is a sizable region distinguished by its flora, fauna, soil, and climate. Temperature and precipitation will be present in biome in an area.
To learn more about biome, refer to the link:
https://brainly.com/question/11491362
#SPJ1
The insoluble fiber that provides the main structural component of plant cell walls is:________
Answer:
the answer is cellulose
Consider one of the procedures where amylase and starch are reacting. If there is strong amylase activity and it is breaking down starch, then you would assign an enzyme activity level of ________.
Consider one of the procedures where amylase and starch are reacting. If there is strong amylase activity and it is breaking down starch, then you would assign an enzyme activity level of 3.
We can describe enzymes as biological catalysts that are actively involved in speeding up a reaction by lowering its activation energy. Amylase is also an enzyme whose function is to break starch into smaller carbohydrates.
The activity level of an enzyme depends on the conditions that are provided to an enzyme. The levels of enzyme activity can be categorized into:
no enzyme activity ------- level 0
low enzyme activity ------ level 1
moderate enzyme activity -------- level 2
high enzyme activity ------ level 3
In the scenario above, the activity of the enzyme amylase is strong as it is actively involved in breaking down starch. This means that the enzyme is working at optimal conditions and has high enzyme activity so the activity level will be 3.
To determine more about enzyme activity, click here:
brainly.com/question/14450547
#SPJ4
What type of membrane protein binds a specific molecule, bringing about a change in shape of the protein, which causes a cellular response? insulin is a hormone that binds to this type of protein.
The type of membrane protein that binds a specific molecule, bringing about a change in the shape of the protein, which causes a cellular response is receptor protein.
In the field of science, receptor proteins can be described as proteins that bind to a specific molecule or ligand, so that a specific cellular response can be produced. The cell membrane has a variety of membrane proteins in order the functioning of the cell. Each receptor protein is specific for a particular ligand.
When a ligand binds to a specific site at a receptor, then certain conformational changes occur in the receptor proteins which causes the cell to produce a response. A receptor can have different 'ligand' binding sites' for different ligands.
The insulin receptors are made up of 2 α and 2 β subunits and they can be seen in the membrane of cells such as liver and muscle cells.
To learn more about receptor protein, click here:
https://brainly.com/question/1496473
#SPJ4
What is the most significant limitation that Carl Schurz's work has as a resource for a historian?
A. Schurz was too young to be a reliable witness.
B. Schurz was not an eyewitness to the events he describes.
C. Schurz wrote his account about fifty years after the events occurred.
D. Schurz was a government official likely biased against the revolution.
Answer is C. Schurz wrote his account about fifty years after the events occurred
The most significant limitation that Carl Schurz's work has as a resource for a historian is that he wrote his account about fifty years after the events occurred.
Carl Christian Schurz was an American statesman, author, and reformer. He immigrated to the United States after the German Revolution of 1848 and quickly rose to prominence within the newly formed Republican Party. He wrote a number of works, such as his Reminiscences (1907–09), a volume of talks, and a two-volume biography of Henry Clay (1887). According to the inquiry, his work is limited by the time interval between the actual incident and the time he ultimately wrote it down.
Because a good eyewitness account necessitates documenting events as they happen, this was a significant limitation. The narratives of occurrences are typically more accurate and in-depth when they are recorded according to how they occur.
To learn more about Carl Schurz visit: https://brainly.com/question/28603655
#SPJ1
9th Grade Biology
In your food web, decomposers and sunlight are not included. Draw each one into your food web and explain their role in an ecosystem.
Decomposers organisms are in charge of decomposing organic matter and returning it to the ground as inorganic elements. Sunlight provides energy to autotroph organisms that use it to produce organic matter.
What is the role of sunlight and decomposers on the trophic webs?
The trophic web is the interaction between different organisms involving the transference of energy when some of them feed on the other ones. The ones placed at lower levels pass energy to the ones at the higher levels.
Different levels compose the trophic web.
Producers Primary consumers are herbivores Secondary consumers are carnivores Decomposers
The role of sunlight is to provide energy to producers. Part of the energy that reaches the Earth's surface is taken by autotroph organisms that use it to produce organic matter from inorganic elements.
Decomposers are microorganisms or small invertebrates in charge of recycling elements from dead organic matter and returning them to the ground as inorganic elements.
In this way, natural cycles begin again passing elements from the ground to autotroph organisms, heterotrophs, decomposers, and finally returning to the ground.
In the trophic web,
the arrow that leaves from sunlight must reach green algae, quaking aspen, wheatgrass, gray willow, and pond lily. This is because sunlight is used by autotrophs. arrows leaving from all the organisms in the trophic web must reach decomposers because decomposers take energy from every organism.You will learn more about trophic webs at
https://brainly.com/question/28341774
https://brainly.com/question/516534
#SPJ1
what is the correct order of steps in a gram staining procedure? 1. iodine is added as a fixative or mordant. 2. the specimen is treated with a stain such as safranin or fuchsin (a pink stain). 3. the specimen is flushed with alcohol to dehydrate peptidoglycans and trap the stain. 4. the specimen is treated with a stain called crystal violet.
The correct order of steps in a gram staining procedure is:
(4) the specimen is treated with a stain called crystal violet.
(1) iodine is added as a fixative or mordant.
(3) the specimen is flushed with alcohol to dehydrate peptidoglycans and trap the stain.
(2) the specimen is treated with a stain such as safranin or fuchsin (a pink stain)
Gram staining is a common staining technique which helps to classify the bacteria into two categories: Gram positive and Gram negative. Gram positive bacteria appear violet in color and Gram negative bacteria appear to be pink. This color difference appears due to the difference in the bacterial cell walls.
To know more about Gram staining, here
brainly.com/question/14969595
#SPJ4
Performing+intervals+of+2+minutes+of+80%+(work+phase)+intensity+followed+by+2+minutes+of+40%+intensity+(recovery+phase)+primarily+target+which+metabolic+energy+system?
Performing intervals of 2 minutes of 80% work phase intensity followed by 2 minutes of 40% intensity recovery phase primarily target which metabolic energy system?
Glycolysis
Natural chemicals in the brain that produce effects similar to those of morphine and other opium-derived drugs are called.
Natural chemicals in the brain that produce effects similar to those of morphine and other opium-derived drugs are called endorphins.
Endorphins- When we experience pain or stress, our bodies create molecules called endorphins. They are also released during enjoyable activities including sex, eating, massages, and exercise. Endorphins aid in pain relief, stress reduction, and overall wellbeing.
Morphine- The morphinane alkaloid known as morphine is a very strong opiate analgesic and psychotropic medication. Morphine has a direct analgesic effect on the central nervous system (CNS), which makes it a strong drug of abuse with a high potential for tolerance and physical and psychological dependency that set up quickly.
To know more about the Endorphins, click on the below link,
https://brainly.com/question/10951896
#SPJ4
Which statement best describes the relationship between photosynthesis and cellular respiration? O Photosynthesis removes carbon from the atmosphere, and cellular respiration releases carbon back into the atmosphere. © Photosynthesis is part of the carbon cycle, and cellular respiration is not. O Only plants perform photosynthesis, and only animals perform cellular respiration. 0 )Photosynthesis releases carbon into the atmosphere, and cellular respiration removes carbon from the atmosphere
The relationship between photosynthesis and cellular respiration is photosynthesis removes carbon from the atmosphere, and cellular respiration releases carbon back into the atmosphere.
The Photosynthesis is the process by which green plants and certain organisms convert light energy into chemical energy. During this process in green plants, the light energy is captured and it is used to convert water, carbon dioxide, and minerals into oxygen and energy-rich organic compounds.
Cellular respiration occurs in the mitochondria of plants, it is the process which breaks down sugar in the presence of oxygen to release energy in the form of ATP. This process also releases carbon dioxide and water as waste products.
Thereby, photosynthesis removes carbon from the atmosphere, and cellular respiration releases carbon back into the atmosphere.
To learn more about photosynthesis refer here
https://brainly.com/question/902771
#SPJ9
Please help!
Write the name of the r pyramid on the line above each pyramid. Then, below, explain the relationships among trophic levels that are shown by the pyramids.
The pyramids represents the hierarchical relationship between the different organisms at various trophic level represented in the form of ecological pyramids.
What do you mean by Ecological pyramids?Any of the progressively higher tiers in a food chain, where consumers are found at the primary, secondary, and tertiary levels and producers are at the bottom. Detritivores, commonly referred to as decomposers, are occasionally believed to have their own trophic level.
1.First Trophic Level: Autotrophs.
2. Second Trophic Level: Primary Consumers.
3. Third Trophic Level: Secondary Consumers.
4. Fourth Trophic Level: Tertiary Consumers.
5. Fifth Trophic Level: Quaternary Consumers
To know more about Trophic Level visit:
https://brainly.com/question/9636698
#SPJ9
HELP ASAP
why is the crust of earth located above the mantle?
Answer:
C.....the crust is less dense than the mantle