high efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

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Answer 1

The efficiency of genetic transformation of intact Saccharomyces cerevisiae cells rose to more than 1 105 transformants per microgram of vector DNA and to 1.5% transformants per viable cell utilising a method that produces competent cells using LiAc.

What is nucleic acids ?

Biopolymers and macromolecules called nucleic acids are necessary for all known forms of life. They are constructed of monomers called nucleotides, which are composed of a nitrogenous base, a phosphate group, and a 5-carbon sugar. Deoxyribonucleic acid and ribonucleic acid are the two primary types of nucleic acids.

Gene expression, cellular activity regulation, genetic information storage and transfer are the three primary uses of nucleic acids.

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Related Questions

sea urchins are broadcast spawners: they release their gametes into the water without any courtship. which of these reproductive isolation mechanisms is most likely affecting sea urchins? group of answer choices mechanical isolation gametic isolation behavioral isolation habitat isolation

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As sea urchins are the broadcast spawners: they all release their gametes into the water without any courtship so they have the gametic isolation

Sea urchins are the spiny, globular echinoderms in the class of Echinoidea. About the 950 species of the sea urchin live on the seabed of the every ocean and that inhabit every depth zone — from the intertidal to seashore they down to 5,000 meters. The spherical, hard shells of the sea urchins are about round and the spiny, that ranging in diameter from the 3 to 10 cm

The Gametic Isolation: the Reproductive Isolation where the mating does occur, but the male and the female gametes which cannot be bind to form the zygote. For example, the surface proteins on the eggs of one of the species prevent the sperm of the wrong species from the entering.

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During which weeks in the moon’s cycle does the moon appear to be getting smaller? (1 point)
A. week 3 and week 4
B. week 1 and week 4
C. week 1 and week 2
B. week 2 and week 3 pls help I need this

Answers

The answer is A because that’s when the cycle restarts

which type of amino acid would you expect to find in large numbers in a protein found in a deep-sea hydrothermal vent (a very hot environment)?

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The type of amino acid that is expected to be found in large numbers in a protein found in a deep-sea hydrothermal vent is called Cysteines.

What is hydrothermal vent?

The hydrothermal vent is defined as an outlet that is located at the base of the sea which allows the release of underground heated water.

Cysteines is the type of amino acid that doesn't denature when subjected to high temperature.

Therefore the type of amino acid that is expected to be found in large numbers in a protein found in a deep-sea hydrothermal vent is called Cysteines

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the experiments of francesco redi used the scientific method to prove the idea of spontaneous generation. disproved that maggots and flies were related. determined that fly larvae were present in raw meat, and when left on the counter they turned into flies. disproved the idea of spontaneous generation. disproved the scientific method.

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The experiments of Francesco Redi disproved the idea of spontaneous generation. The correct option is D.

Who is Francesco Redi?

Francesco Redi, an Italian physician and poet, demonstrated that the presence of maggots in putrefying meat is not due to spontaneous generation, but rather to flies' eggs laid on the meat.

Redi concluded that the maggots were caused by flies laying eggs on the meat in the open jar.

Maggots were not produced because the flies were unable to lay eggs on the meat in the covered jar.

As a result, Redi demonstrated that decaying meat did not produce maggots.

Francesco Redi's experiments disproved the concept of spontaneous generation.

Thus, the correct option is D.

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which question is most closely related to the feild of biology

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The question most closely related to biology is, what influences the fish population's growth rate?

What kind of subject is biology?

The study of living things and their essential functions is the subject of the science known as biology. Botany, conservation, ecology, evolution, genetics, marine biology, medicine, microbiology, molecular biology, physiology, and zoology are only a few of the many disciplines included under the umbrella term "biology."

Books in this topic area are on biology, which is the study of living things. It focuses more specifically on all living organisms' structure, function, growth, origin, evolution, distribution, and classification.

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The prevalence of genetically predisposed traits that have a reproductive advantage is best explained in terms of.

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The prevalence of genetically predisposed traits that have a reproductive advantage is best explained in terms of natural selection.

What is natural selection?

Natural selection is the process of selecting only dew traits to go to the next generation. Those traits are selected that are better and that can withstand future conditions.

It is a genetic process which is done naturally by the environment.

Thus, Natural selection provides the best explanation for the prevalence of genetically predisposed features that are advantageous for reproduction.

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a(n) is a compound that is produced in one tissue, released into circulation, then has an effect on a different organ.

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The "hormone" is that compound which produced in one tissue, released into circulation, then has an effect on a different organ.

A family of signaling molecules known as hormones is delivered from distant organs in multicellular animals to control physiology and behavior. The proper development of fungi, plants, as well as animals depends on hormones.

Hormones are directly released into the bloodstream by endocrine glands. Numerous bodily processes, including growth, repair, and reproduction, are regulated by hormones. Numerous organ systems including hormones, including several that have been currently being studied and understood, were part of the endocrine system.

An agonistic relationship exists when one hormone would be required for the effects of a second hormone to manifest.

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2017 schulick endoplasmic reticulum (er) stress are jointly expressed in idiopathic pulmonary fibrosis (ipf)

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There exist a link between lung fibrosis and stress on the endoplasmic reticulum.

Many fibrotic diseases such as Idiopathic Progression Fibrosis are related to the stress on the endoplasmic reticulum. The discovery of the first case of ER stress was fifteen years ago along with the discovery of a mutation in protein C. With apoptosis of alveolar epithelial cells, differentiation of fibroblast and polarization M2 macrophage, lung fibrosis, and ER stress is linked with each other.

Various analyses and tests are being performed to understand the effects of ER stress and the factors that result in ER result in cells such as macrophages, alveolar epithelial cells, and fibroblasts. So, there exists a link between lung fibrosis and stress on the endoplasmic reticulum.

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based on cell size and complexity, prokaeyotic cells would be expected to

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based on cell size and complexity, prokaryotic cells would be expected to have fewer ribosomes.

What is the function of ribosomes?

Any cell's ribosome produces proteins as its primary function. Almost all biological processes involve the utilization of proteins; they operate as catalysts to speed up activities; as fibers, they give support; and many proteins perform specific jobs, such as contracting muscle cells. DNA, or deoxyribonucleic acid, is the precursor to all proteins. A unique protein called RNA polymerase is an enzyme that can identify DNA sequences, bind to them with the assistance of other proteins, and produce a new information molecule that can go from the cell's nucleus to its cytoplasm. On its way out of the nucleus, the strand of ribonucleic acid (RNA) created by RNA polymerase is processed, and regions of the RNA that do not code for proteins are deleted. The molecule is now referred to as an mRNA, or messenger RNA.

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What roles do membrane proteins play in transporting only certain substances into a cell.

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Some allow very specific molecules to pass, even against their concentration gradient. Others work as a one way control. Others let many things go through role do membrane proteins play in transporting only certain substances into a cell.

A molecule is two or more atoms held together by chemical bonds that form the smallest unit of matter that holds its composition and properties. Molecules shape the idea of chemistry. Molecules are marked with element symbols and atomic number subscripts. Atoms are the fundamental devices of elements. They consist of an atomic nucleus and surrounding electrons.

If an atom has an incomplete electron cell, it is said to have valence electrons. When two or more atoms come together to share their outer shell valence electrons, they form a chemical (covalent) bond and enter a lower energy state. When atoms combine, energy is released in an exothermic reaction. When a covalent bond is broken and the molecule splits, an energy input is required, thus making it endothermic.

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A woman brings in a spray puppy she has rescued off the street. She wants to adopt the dog.
but it looks sick. The dog seems tired, will not eat, and has diarrhea. Dr. Snyder looks very concerned. He takes a small sample of the puppy's feces and runs a test. Fifteen minutes later he comes back with bad news: the puppy has parvovirus. The disease is often fetal, but many dogs survive with good treatment.
Dr. Snyder tells Lizzie that parvovirus is preventable, and a dog cannot get infected if it is given a shot.
What type of shot would prevent a dog from the parvovirus disease? Explain how this shot protects a
dog so it cannot get sick from a virus.

Answers

Answer:

DHPP Vaccine

Explanation:

is used to protect your pet

a forest is cut down to make room for a housing development. which population is most likely to survive? question 5 options: raccoons, which can eat a variety of foods woodpeckers that need to nest in a particular tree large predators that need to hunt over a wide area salamanders that hibernate in rotting logs

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Raccoons can eat a variety of foods, according to the situation that forest is cut down to make room for a housing development, so this  population has the best chance of survival. The correct option is A.

What is specialist species?

Specialist species are the ones that require very particular resources. These lifeform frequently have a limited diet or necessitate a proper habitat condition to stay alive. Specialists encompass tigers salamanders.

A generalist species can thrive in a long area of environmental conditions as well as utilize a wide range of resources for illustration, a heterotroph with a varied feeding habits.

A specialist species can only thrive in a limited range of environmental conditions or generally has a restricted diet.

Raccoons can eat a variety of foods based on their respective environment. This population has the greatest chance of sustaining life as the forest is cut down to make room for a housing development..

Thus, the correct option is A.

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Write a grammatically correct sentence that compares that reactants and products of respiration

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The reactant involved in cellular respiration is glucose molecule and six oxygen molecules, while the products are six molecules of carbondioxide and six molecules of water respectively. The chemical equation is

C6H12O6 + 6O2 → 6CO2 + 6H2O

glucose + oxygen -> carbon dioxide + water

Cellular respiration is a catabolic process thus the reaction is exothermic yielding energy in the form of ATP.  Cellular respiration is a process that spans through glycolysis, tricarboxylic acid cycle and the electron transport chain in supply the cells of the body with requisite energy for getting work done. The reverse reaction of cellular respiration is referred to as photosynthesis.  

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various terms denote chains of amino acids based on the size of the chains. put the terms protein, peptide, and polypeptide in order from smallest at the top to largest at the bottom.

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The correct order is peptide, polypeptide, Protein.

What is Protein?

Large molecules and macromolecules known as proteins are made up of one or more extended amino acid residue chains. Among the many tasks that proteins carry out in living things include catalyzing metabolic processes, replicating DNA, reacting to stimuli, giving cells and organisms structure, and moving molecules from one place to another.

The primary way that proteins differ from one another is in the order of their amino acids, which is determined mostly by nucleotide sequence of their genes and typically causes a protein to fold into a certain 3D structure that controls its activity.

A polypeptide is an ordered sequence of amino acid residues. At least one lengthy polypeptide is present in every protein.

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The chains of amino acids from smallest to largest-

- peptide

- polypeptide

- protein

What are amino acids?

Organic substances known as amino acids have both functional groups for amino and carboxylic acids. Alpha-amino acids, which make up proteins, are by far the most common amino acids in nature despite the fact that there are hundreds of them. The genetic coding only contains 22 alpha amino acids.

The locations of the primary structural functional groups in amino acids can be categorized as alpha, beta, gamma, or delta amino acids; further classifications relate to polarity, ionization, and the type of side chain group (aliphatic, acyclic, aromatic, containing hydroxyl or sulfur, etc.).

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Suggest why farmers in low-income countries may not use selective herbicides

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Answer:

Many farmers in developing countries view pesticide use as the best means to

protect their crops against pests, such as desert locusts in parts of Africa this year, which are often the main

threat they face. As such, pesticides can provide the only form of crop insurance available.

Explanation:

magro-malosso er, saccone g, di tommaso m, et. al.: neuraxial analgesia to increase the success rate of external cephalic version: a systematic review and meta-analysis of randomized controlled trials. am j obstet gynecol 2016; 215: pp. 276-286.

Answers

The correct answer is neuraxial analgesia improves the success rate of external cephalic version in women with malpresentation at term or late preterm, which increases the likelihood of vaginal delivery.

The use of neuraxial analgesia improves the success rate of external cephalic version in women with malpresentation at term or late preterm, which increases the likelihood of vaginal delivery. The brain and spinal cord make up the central nervous system (CNS). The use of local anaesthetic in or around the CNS is referred to as neuraxial anaesthesia. Spinal anaesthesia is a type of neuraxial anaesthesia in which a local anaesthetic is administered directly into the intrathecal space (subarachnoid space). Neuraxial analgesia can be used to relieve labour pain and provide an in-situ epidural catheter for rapid conversion to surgical anaesthesia for operative delivery if necessary.

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37) This Doppler radar instrument transmits electromagnetic energy in the form of microwaves. Some
microwave wavelengths are between the wavelengths of
1) gamma rays and x rays
(2) Infrared and radio waves
(3) ultraviolet and infrared
(4) x rays and ultraviolet

Answers

The purpose of weather radar, often referred to as Doppler weather radar, is to locate precipitation, assess its velocity and intensity, and determine the precipitation type, such as rain, snow, or hail. It does this by sending electromagnetic energy pulses into the sky.

Doppler radar may detect the mobility of the rain along the radar beam in addition to the precipitation in a rainstorm (by its reflectivity, or capacity to reflect microwave radiation). To put it another way, it can calculate how quickly hail or rain is travelling towards and far from the radar. The electromagnetic waves used by radar move at a rate of 300,000,000 m/s. A local TV news station's weatherman uses Doppler radar, for instance, to predict storms. A police officer might utilise Doppler radar to gauge the speed of an approaching car as an illustration. Astronomers might use Doppler radar to gauge the speed of a moving star as an illustration.

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cartilaginous fish (chondrichthyes): have a swim bladder. have teeth mineralized by calcium phosphate. are generally freshwater species. have well-developed kidneys.

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Cartilaginous fish Chondrichthyes have teeth mineralized by calcium phosphate. The correct option is B.

What are Chondrichthyes?

Chondrichthyes is a class of cartilaginous fishes, which have skeletons made primarily of cartilage.

They differ from the Osteichthyes, or bony fishes, which have skeletons made mostly of bone tissue.

Calcium phosphate mineralizes the teeth of cartilaginous fish. Mineralization of teeth is an operation in which living organisms incorporate cellular material with environmental minerals.

This process occurs to form a matrix structure, which usually results in harden structures such as tooth enamel or bone matrices.

Thus, the correct option is B.

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chlorella and paramecium are both unicellular eukaryotic organisms living in freshwater. chlorella is photosynthetic and has a cell wall. which organelle will be found in chlorella but not the paramecium?

Answers

Contractile vacuole is an organelle will be found in chlorella but not the paramecium.

The lowest metazoans, such as sponges and hydras, and freshwater protozoa have regulating organelles called contractile vacuoles that gather extra fluid from the protoplasm and occasionally release it into the surrounding media. Wastes containing nitrogen may also be excreted.

The contractile vacuole (CV) complex is an osmoregulatory organelle found in free-living amoebae and protozoa that regulates intracellular water balance by storing and excreting excess water, enabling cells to survive under hypotonic stress, like that found in pond water.

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Put the following events into the correct order in which they are thought to have occurred according to the impact hypotethesis

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Here is the order of events which they are thought to have occurred according to the impact hypothesis.

impactdebris and tsunami devastate North Americasoot released by global forest fireslong global winterextinction of large fraction of all species

The moon may have formed from the ejecta of a collision between the proto-Earth and a planet the size of Mars around 4.5 billion years ago, according to the giant-impact hypothesis, also known as the Big Splash or the Theia Impact (about 20 to 100 million years after the Solar System coalesced).

Theia, the name of the fabled Greek Titan who was Selene, the goddess of the Moon's mother, is frequently used to refer to the colliding bodies. According to a 2016 report on lunar rock analysis, the impact may have been a direct hit, thoroughly mixing both parent planets.

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proteomics differs from genomics because proteomics concerns the complete set of an organism's proteins.

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Proteomics and genomics are topics that interact frequently. The primary distinction between genomics and proteomics is that the former studies the whole set of genes in a cell's genome, while the latter studies the entire set of proteins the cell produces.

Through the genetic code, the nucleotide sequence of a gene dictates the amino acid sequence of a protein. In order to make the appropriate proteins, mRNA is translated from genes into mRNA The total expressed proteins in an organism are represented by the proteome. Proteomics refers to the field of research that identifies the traits, structures, functions, and expressions of every protein in a cell.

Understanding the structure, function, location, and regulation of an organism's genes requires genomic studies. Since proteins are the actual functional molecules in cells and represent true physiological conditions, proteomics investigations are more advantageous.

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you need vitamins to use the carbohydrates, fats, and proteins you consume and to sustain numerous chemical reactions in your body.

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True, B vitamins help human bodies utilize minerals, produce new red blood cells, use carbohydrates, protein, and fat, and maintain a healthy nervous system. The three most well-known B vitamins are thiamin, riboflavin, and niacin. Everybody has to take B-Complex vitamins every day.

Thiamin- Vitamin B1

Riboflavin- Vitamin B2

Niacin- Vitamin B3

Red Blood Cells (RBC)- Red blood cells are the cells responsible for the oxygen transport to all the cells of the living organism. RBC have hemoglobin which is responsible for red color in blood.

B-Complex vitamins contains 12 different types of Vitamin B which also includes, B1, B2, and B3.

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Do the winds in Paris bring a seasonal change

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Over the course of the year, the temperature typically varies from 35°F to 78°F and is rarely below 25°F or above 88°F.

The best description of the greenhouse effect is that it _______. a. is solely the result of burning fossil fuels b. generates heat in the atmosphere c. traps the heat generated by burning fossil fuels d. collects heat emitted by the sun please select the best answer from the choices provided a b c d

Answers

Answer:

D

Explanation:

When your muscles contract to move your body, such as during walking, work is done, and work requires energy. Based on the law of conservation of energy, from where does this energy come?.

Answers

The breakdown of ATP provides the energy needed for muscle contraction, however, the amount of ATP present in muscle cells is only enough to power a brief period of contraction.

Because it breaks the myosin-actin cross-bridge and releases the myosin for the following contraction, ATP is essential for muscle contractions.

Actin and Myosin muscle fibers are worked on by releasing the chemical potential energy contained in bonds within ATP, and then work is required to rebuild those bonds.During cross-bridge cycling, ATP is hydrolyzed, converting chemical energy into mechanical energy during muscle contraction. This mechanical energy is then distributed throughout the tissue and stored there as the muscle contracts or is used for an external effort.Adenosine triphosphate (ATP), the body's biochemical means of storing and transporting energy, is the source of energy that is used to drive the movement and contraction of working muscles.Adenosine triphosphate (ATP), found in muscles, provides energy. ATP levels in muscles are typically quite low. Creatine phosphate (CP) and muscle glycogen must be used to recreate ATP when it is low.

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blood, which is made up of multiple cell types including red and white blood cells, is an example of a(n)

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The blood, which is made up of multiple cell types including red and white blood cells, is an example of a tissue.

What is a tissue?

A tissue can be defined as a group of specialized cells that emerges by differentiation and work together to perform a given task (or more than only one) in the body.

In conclusion, the blood, which is made up of multiple cell types including red and white blood cells, is an example of a tissue.

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A scientist isolated DNA from a particular bacterium and decided to analyze it. The sequence of the bacterial DNA is ATGGGCTASTCTT What will
the complementary strand be?

Answers

complementary strand of   ATGGGCTASTCTT will TACCCGATAGAA

Base Pairing is a principle that bonds in DNA can form only between adenine and thymine and between guanine and cytosineReplication is process of copying DNA prior to cell divisionDNA Polymerase  copys DNA prior to cell divisionTransformation is process in which one strain of bacteria is changed by a gene or genes from another strain of bacteriagene is section of DNA that codes for a specific traitgenome is the entire "library" of genetic instructions in DNA that an organism inheritsgenotype is the combination of alleles located on homologous chromosomes that determines a specific characteristic

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Who was G. Stanley Hall and what did he mean by "storm and stress?"

Answers

Answer: The Father of Adolescence

Explanation:  G. Stanley Hall, came up with the term 'storm and stress' to describe adolescence in 1904. He referred to an adolescent's decreased level of self-control as the 'storm' and their increased sensitivity as the 'stress'.

the ability of the kidneys to remove molecules from the blood plasma by excreting them in the urine is known as .

Answers

the ability of the kidneys to remove molecules from the blood plasma by excreting them in the urine is known as Glomerular filtration. one can estimate the efficiency of Glomerular by GFR Glomerular filtration rate

Measuring measured GFR (mGFR) is a difficult and time-consuming method that is not practicable for patients or doctors, making it difficult to obtain an accurate GFR level. Because of this, medical experts utilise a formula to determine GFR (eGFR). For diagnosing renal disease, which frequently has no symptoms until right before the kidneys fail, accurate estimates of GFR are crucial. A straightforward blood test that evaluates your creatinine levels is the typical method for estimating GFR. The regular breakdown of muscle tissue and the digestion of food protein both produce creatinine, a waste product. In addition to CKD, other factors such as food, muscle mass, or the weight of your muscles, malnutrition, and other chronic conditions can also have an impact on creatinine levels.

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The concentration of solute is less on one sides of the membrane causing water to move into the cell causing the cell to swell.

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When a cell is immersed in a hypotonic solution, the volume of the cell will rise as it will cause will be a net inflow of water inside the cell. If the concentration of solutes outside the cell is lower as compared to inside the cell and when the solutes are not able to cross the membrane, then the solution is called hypotonic to the given cell.

The ability of an extracellular solution to induce water to osmotically enter or depart a cell is known as tonicity.

Three terms—hypertonic, hypotonic, and isotonic—are employed in order to evaluate if a solution would cause water to move into or out of a cell:

If a cell is immersed in a hypertonic solution, it will lose volume because there will be a net flow of water out of the cell.A cell will shrink if it is submerged in a hypertonic solution because water will escape the cell. Since there is no net water movement in an isotonic environment, the cell's size remains constant. A cell will absorb water and swell when it is exposed to a hypotonic environment.

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One pastime that is enjoyed around the world is watching movies and going to the movies! Let's use our preterite past tense skills and pastimes vocabulary knowledge to talk about the last movie we saw with a group of people. Include as many simple details as you can.Please respond to the following prompts.Escribe sobre la ltima vez que viste una pelcula con un grupo de personas. Incluye tantos detalles como sea posible. (Dnde? Con quin, cundo? Cul pelcula? Por qu?) Example: La ltima vez que fui al cine fue a ver ______. Yo vi la pelcula ____ con _____. Fuimos a ______. Comimos_____. etc.Please respond to all of the above questions in complete sentences and respond in simple Spanish sentences using the vocabulary we are learning and your prior knowledge Can you have a triangle with sides 10, 11, and 12? 3. Jackson says that, for him, the best poems are which ones? PLEASE HELP .!!! ILL GIVE BRAINLIEST.. *EXRTA POINTS* .. DONT SKIP :(( ! ILL GIVE 30 POINTS . if each value of the data of the poplation function is doubled which of the following is not correct?A. the mean is doubledB. the mode is doubledC. the variance is doubledD. the median is doubled A student is collecting the gas given off from a plant in bright sunlight at a temperature of27C. The gas being collected is probablyA) oxygenB) carbon dioxide) OD) glucose Describe the structure of the cell membrane. 51. The radius of gold is 144 pm and the density is 19.32 g/cm3. Does elemental gold have a face-centered cubic structure or a body-centered cubic structure Why were some of the Achievements of the progressive era Which of the following hormones are related to mood changes? Select all that apply.thyroxineestrogensmelatoninaldosterone 5Note that is the only solution to the equation 12y - 7 = 3. Which of the following is the solution set of the equation 12y - 7 = 3?O [5,6] {{}0560 y = /1/201{}}O a pyramid is made of metal balls. the lowest layer consists of 4 by 4 balls ,the next layers of 3 by 3 and 2 by 2 and one ball at the top. every two balls that touch each other are connected with screw. how many screws are used to build this pyramid? why is the Gautrain famous What is the relationship between the Talmud and the Hebrew Bible? What do they say about Jewish ideas of righteousness and justice?NO go-og-le answer! Rewrite each of the sentences below correcting it so there are no double negatives.Remember that there is often more than one way to correct each error. 8. I haven't never seen no marsupials, even on television.9. My grandmother Sanchez hasn't never left her beautiful homeland of Puerto Rico.10. Soon-Yi doesn't have no easy answers to that complicated problem.11. We don't need no education.12. I can't get no satisfaction.13. They can't hardly run the required distance.14. Harry doesn't have no reason to complain.15. The seagulls do not ever resist the waste receptacles at the beach.16. Our choir members can't scarcely hear the director's cue. Reduce completely![tex]\frac{3*3*4*4}{3*4*5*6}[/tex] Joan Arlington has twice as much money invested at 5% simple annual interest as she does at 4%. If her yearly income from the two investments is $154, how much does she have at each rate? equation Europeans set up plantations primarily to? Select the correct answer.Why is the 17th Amendment significant?A. It upholds the antitrust of the Northern Securities Company.B. It requires the direct election of US senators by voters.C. It allowed women to vote.D. It spelled out illegal practices for businesses.E. It investigated violations of federal regulations and found unfair ways of competition. In a class of 30 students, the mean height of the 12 girls is 164 cm and the mean height of the 18 boys is 166 cm. Find the mean height of the whole class.