Perform a DNA extraction from green peas at home or school lab, under adult supervision, by following the given procedure carefully.

Procedure for DNA extraction:

To half cup of split peas, add 1/8 teaspoon of salt and one cup of cold water.
Transfer this mixture in a blender and blend it on high speed for 15 seconds.
Filter the blended mixture, using a strainer, into a measuring container. Observe the amount of mixture you obtain after filtration. Add liquid detergent equal to 1/6 volume of the obtained pea mixture.
Mix by swirling. Allow the mixture to rest for about five to ten minutes.
Transfer this mixture to test tubes. Fill the tubes to about 1/3 of their volume.
Add a pinch of meat tenderizer to each of these tubes. Mix gently to avoid damaging the DNA.
Tilt the test tube, and pour alcohol (75% isopropyl alcohol or 95% ethanol) down the sides of the tube. Add alcohol to about 2/3 volume of the tube. You will obtain two layers. The layer at the top contains the DNA.

Now that you are done with the DNA extraction process, answer the following questions:

What purpose does blending the pea mixture serve?
Why do you need to add liquid detergent?
What role does the meat tenderizer play?
Why is alcohol added and why does the DNA get extracted in the alcohol layer?

Answers

Answer 1

Answer:

Blending is done to break open, or lyse, the cells.

DNA resides in the nucleus, which is enclosed by the nuclear membrane. The nucleus is further enclosed by the cell membrane. So, to get the DNA, we need to break open both the membranes. Cell membranes are made up of lipids and proteins. The detergent molecules to attach to the cell and nuclear membranes, causing them to break open.

Meat tenderizer contains the enzyme protease that helps cleave proteins. Even after breaking open the nucleus and cell membrane, the DNA is still protected by cell proteins. Meat tenderizer cleaves the proteins, leaving behind only the DNA molecule.

DNA is insoluble in alcohol and hence, when alcohol is added, it precipitates out the DNA.

Explanation:

PLATO Answer


Related Questions

One litre of hydrogen at STP weight 0.09gm of 2 litre of gas at STP weight 2.880gm. Calculate the vapour density and molecular weight of gas. ​

Answers

Answer:

we know, at STP ( standard temperature and pressure).

we know, volume of 1 mole of gas = 22.4L

weight of 1 Litre of hydrogen gas = 0.09g

so, weight of 22.4 litres of hydrogen gas = 22.4 × 0.09 = 2.016g ≈ 2g = molecular weight of hydrogen gas.

similarly,

weight of 2L of a gas = 2.88gm

so, weight of 22.4 L of the gas = 2.88 × 22.4/2 = 2.88 × 11.2 = 32.256g

hence, molecular weight of the gas = 32.256g

vapor density = molecular weight/2

= 32.256/2 = 16.128g

hence, vapor density of the gas is 16.128g.

Explanation:

can someone help me identify the elements please, i rlly need help

Answers

Answer:

1 : any of more than 100 substances that cannot by ordinary chemical means be separated into different substances Gold and carbon are elements. 2 : one of the parts of which something is made up There is an element of risk in surfing. 3 : the simplest principles of a subject of study the elements of arithmetic.

Explanation:

Which of the following is an extensive property of a sample of aluminum?

Answers

Answer:

Entropy,mass and volume.... I think

Mass is the extensive property of a sample of aluminum. Therefore, option (A) is correct.

What is the extensive property of matter?

The extensive property can be described as a physical property of matter that changes with a change in the size and shape of the matter. Therefore, the extensive property of any substance varies directly with the mass.

Extensive properties are the value of the property of the system that must be equal to the sum of the values of different parts of the system. These properties depend on the amount of matter contained in the system.

Examples of Extensive properties are temperature, pressure, density, boiling point, etc. The ratio of the two extensive properties is an intensive property such as density.

The mass of a sample of aluminum is an example of extensive property as it depends on the amount of aluminum in the given sample. This property of the sample is proportional to the size of the system.

Learn more about the extensive property, here:

https://brainly.com/question/13733851

#SPJ6

Your question is incomplete, most probably the complete question was,

Which of the following is an extensive property of a sample of aluminum?

A) Mass

B) Temperature

C) Pressure

D) Density

HELP!!!!!! WILL MARK BRAINLIEST!!!


Fill in the blank.

Atoms are held together in molecules by _______.
_______ are compounds with the same chemical formula but different molecular structures.
A/An _______ indicates the ratio of atoms in a molecule.
_______ are similar to molecular formulas but show how atoms are bonded together.
When two atoms of the same element combine to form a molecule, this is called a _______ molecule.
The breaking and forming of bonds between atoms in substances results in changes in the _______ contained in the substances.
Respond to the following based on your reading.

Give two purposes for the systematic naming of chemicals.
Name the elements in the molecule H2SO4 and tell how many atoms of each element are present.
How many molecules are represented by 3CO2?

Answers

Answer:

1. Covalent Bond

2. Isomers

3. Molecular and Empirical Formula

4. The Structural Formula

5. Diatomic Molecules

1. Hydrogen and Tetraoxosulphate. There are 2 atoms of hydrogen and 4 atoms of tetraoxosulphate.

Explanation:

Sorry, I wrote all I know

:answer question number 10

Answers

Answer:

the last one

Explanation:

I'm pretty sure

Answer:

Particles on each are constantly moving (the last one)

Which element is a metal? A. K
B. Kr
C. F
D. He
please help, if you’re right i’ll give brainliest and 35 more points

Answers

Answer: A

Explanation: Potassium is a metal.

correct answer would be A

The movement of air caused by differences in air pressure is __________________.

Answers

Answer:

wind

Explanation:

it would be naturally caused by the pressure

Answer:

temperature or pressure differences

Explanation:

Susie cooked sausages on a barbecue. Fat and water in the sausages changed state. which two changes of state occur during this process? a. Fat melted b. Water evaporated

Answers

Answer:

The options a and b are correct

Explanation:

This options provided to the question are the answers to the question. But for clarification. When the fat melts, the change of state that occurs here is from solid to liquid (which is called melting) while the change of state that occurs when the water evaporated is from liquid to gas (which is called evaporation).

PLEASE HELP ASAP I EILL GIVE 100 POINTS!!! Kristy is on the track team. She runs the one kilometer race by going four times around the circular track. How does Kristy velocity change.
A. Her velocity changes countinually since her direction constantly changes
B. Her velocity depends on only on how she paces herself during the race
C. Her velocity is the same as her speed
D. Her speed changes at the same rate as velocity

Answers

Answer:A

Explanation:

How do you determine if you have a covalent bond?

Answers

Answer:

If the electronegativity difference (usually called ΔEN) is less than 0.5, then the bond is nonpolar covalent. If the ΔEN is between 0.5 and 1.6, the bond is considered polar covalent. If the ΔEN is greater than 2.0, then the bond is ionic.

Explanation:

Hope this helped :)

Which of the following best describes the Valence Shell Electron Pair Repulsion Theory (VSEPR)?

Answers

Answer:

Electron pairs are arranged as far apart from each other as possible.

Explanation:

Like charges repel therefore, VSEPR theory is the "...repulsions between electron pairs present in the valence shell of the central atom."

Critical thinking : Place an unmagnetized piece of iron in a magnetic field (eg iron filings near a magnet). Why is it attracted to the magnet?

Answers

Magnets attract iron due to the influence of their magnetic field upon the iron. Before a piece of iron enters the magnet, the polarization of the irons atoms is random. When exposed to the magnetic field, the atoms begin to align their electrons with the flow of the magnetic field, which makes the iron magnetized as well. This, in turn, creates an attraction between the two magnetized objects. This is why a piece of iron that is exposed to a magnet becomes magnetic for some time afterward.

can someone tell me how I find the atomic radius of atoms and what they are. Thank youuuuuuuuu

Answers

Answer:

The radius of an atom can only be found by measuring the distance between the nuclei of two touching atoms and then halving that distance. Measuring the atomic radii of chemical elements is a complicated task as the size of an atom is of the order of 1.2×10-10 m. The electron cloud forming the shell of an atom does not have any fixed shape which makes it difficult to determine the atomic size of an atom. So we can say that practically we cannot determine the size of an individual atom.

Atomic Radius:

Atomic radius is the distance from the center of the nucleus to the outermost shell containing electrons.

Explanation:

1000 cm3 of Helium has a mass of 178.5 grams. What is the density of Helium gas?​

Answers

Answer:

The answer is 0.18 g/cm³

Explanation:

The density of a substance can be found by using the formula

[tex]density = \frac{mass}{volume} \\ [/tex]

From the question we have

[tex]density = \frac{178.5}{1000} \\ = 0.1785[/tex]

We have the final answer as

0.18 g/cm³

Hope this helps you

Balance the following reaction. A coefficient of "1" is understood. Choose the option "blank" for the correct answer if the coefficient is "1."
C3H8 + O2 → CO2 + H2O

Answers

C3H8 + 5O2 —-> 3CO2 + 4H2O

Answer:

Hope this helps!

Explanation:

What is the formula for nitrogen monoxide?
O N203
O NO
O N₂O,
O None of the answers are correct

Answers

The answer is NO.

Mono- means 1, so monoxide would indicate that there is 1 oxygen.

The N is double bonded to the O:

N=O

And has an unpaired set of electrons

what are chiral carbon​

Answers

Answer:

A chiral carbon is a carbon bonded to four different groups ( four groups as carbon forms four bonds). The molecules formed are non superimposible on its mirror image.

the person who answered before me is correct!

A high school chemistry teacher asks four student groups to determine the number of protons, neutrons, and electrons (respectively) present in an ion of Titanium (Ti 2+). Which student group provides the best answer?

Answers

Answer:

Electrons - 20

protons - 22

neutrons - 26

Explanation:

Titanium has an atomic number of 22 indicating that the neutral atom has 22 protons.

Ti 2+ has lost two electrons and contains only 20 electrons, 22 protons.

The mass number of titanium is 48. Hence;

Mass number = atomic number + number of neutrons

Since the atomic number is 22 and the mass number is 48

number of neutrons = 48 - 22 = 26 neutrons

What mass of ethanol will release 410.0 J of heat when it cools
from 32.2°C to 19.1°C?
The specific heat of ethanol is 2.44 J/gºC.

Answers

12.8 g

(Don’t have explanation but it is right on CK-12)

What is the temperature of someone with a fever? Select the better estimate.
40°F
40°C

Answers

Answer:

40°C

Explanation:

THIS S VERY HIGH FEVER

AS    40°C  =  104°F

Which of the following most completely describes a redox reaction?
O A. A chemical decomposition reaction
B. A hydrocarbon combustion reaction
O c. A reaction resulting in electron sharing
D. An oxidation and reduction reaction

Answers

Answer:

D.

Explanation:

Because i just copied the person above tbh, and i also got it right in the quiz

To solve such this we must know the concept of redox reaction. Therefore, the correct option is option D that is an oxidation and reduction reaction.

What is chemical reaction?

Chemical reaction is a process in which two or more than two molecules collide in right orientation and energy to form a new chemical compound. The mass of the overall reaction should be conserved. There are so many types of chemical reaction reaction like combination reaction, double displacement reaction.

Redox reaction is a chemical reaction where oxidation and reduction takes place simultaneously. Oxidation is loss of electrons and reduction is gain of electrons. The electron transfer from oxidant to reductant.

Therefore, the correct option is option D that is an oxidation and reduction reaction.

Learn more about the chemical reactions, here:

https://brainly.com/question/3461108

#SPJ2

i have the most potential energy if i am​

Answers

Answer:

travel the world in one day

The repeating subunits that are responsible for the chance of a crystal are know as
unit cells
heavy nuclei
light electrons
amorphous cells

Answers

Unit cells.
They are the smallest group of atoms that take form of a crystal and can repeat in 3 dimensions.

Which of the following is TRUE about when two atoms form a bond? a. Repulsion is greater than attraction b. Neither atoms will have a full valence shell c. Potential energy is at a minimum

Answers

Answer:

b:   Neither atoms will have a full valence shell

Explanation:

I think sooo

5. What do the data in your graphs tell you? Explain in one or two sentences what trend the reader should observe in each of your graphs. (Science Lab Report, if you don't know what the answer is please don't answer-)

Answers

Answer:

Graphs are a common method to visually illustrate relationships in the data. The purpose of a graph is to present data that are too numerous or complicated to be described, and  If the data shows pronounced trends or reveals relations between variables, a graph should be used.

Explanation:

literally bro i had the same answer for my actual project for edge

Answer: Graphs are a common method to visually illustrate relationships in the data. The purpose of a graph is to present data that are too numerous or complicated to be described, and  If the data shows pronounced trends or reveals relations between variables, a graph should be used.

Explanation:

Which list contains only animal phyla? mosses, annelids, conifers, chordates mosses, ferns, conifers, flowering plants cnidarians, mollusks, annelids, arthropods, echinoderms, chordates cnidarians, ferns, annelids, arthropods, echinoderms, flowering plants

Answers

Answer:

cnidarians, mollusks, annelids, arthropods,

Explanation:

Living organisms have been grouped using a system of classification that runs from Kingdom to Species. One of those kingdoms is KINGDOM ANIMALIA, which consists of animal species i.e. organisms that are strictly heterotrophic and eukaryotic.

The next taxa after kingdom is PHYLA. According to this question, the phyla which consists of only animals are cnidarians, mollusks, annelids, arthropods. Other options contain ferns, mosses, and flowering plants which belong to Kingdom Plantae.

Answer:

on edge its C

Explanation:

calculate the weight of 5 atoms of Mg.​

Answers

Answer:

2.0179701e-22

Explanation:

The independent variable and dependent and constant

Answers

Answer:

Explanation:

independent-longer wire

dependent-bulbs being brighter

constants-lamp brand and so on

Would it be possible for electrons in the 2p orbitals to be inside the 2s orbital?
(Please explain)

Answers

Answer:

Yes

Explanation:

You can have 2s and have part of the 2p of the row in the p section.

method used to separate ethanol from propanol​

Answers

Fractional distillation is a method for separating a liquid from a mixture of two or more liquids. For example, liquid ethanol can be separated from a mixture of ethanol and water by fractional distillation. This method works because the liquids in the mixture have different boiling points.
Other Questions
THE EGG! This musical number is an extended metaphor, so it makes a comparison between two unlike objects that compares more than one feature of those objects. Explain what objects/ideas are being compared to each other, and how they are being compared. Your response should be a paragraph of 5 sentences, minimum. Which of these is an example of heat transfer by conduction? cooking carrots in hot waterdrying hair with a blow dryerpressing a shirt with an ironwarming water with sunlight To add or subtract fractions, what must you have? what is one way the United States government dealt with the issue of slavery in the western territories of Kansas and Nebraska? Find the area of the shaded region. The graph to the right depicts IQ scores of adults, and those scores are normally distributed with a mean of 100 and a standard deviation of 15.105 is on the graph WILL MARK BRAINLIEST Short-response prompt (15 points)Read the following passage from Act I, scene ii of The Tempest, in which Prospero summons Caliban.PROSPERO . . . Come on;We'll visit Caliban my sIave, who neverYieIds us kind answer.MIRANDA'Tis a viIIain, sir,I do not love to look on.PROSPEROBut, as 'tis,We cannot miss him: he does make our fire,Fetch in our wood and serves in officesThat profit us. What, h0! sIave! CaIiban!Thou earth, thou! speak.CALIBAN[Within] There's wood enough within.PROSPEROCome forth, I say! there's other business for thee:Come, thou tortoise! when?(. . .)Thou poisonous slave, got by the devil himselfUpon thy wicked danm, come forth!From the passage, identify words and phrases that refer to Caliban. Then, analyze how the choice of words shapes readers' first impression of Caliban and shows Prospero and Miranda's attitude toward him. Be sure to use details from the passage to support your analysis. Which statement would be included in the proof of why Measure of angle C + measure of angle G + measure of angle H = 180 degrees.?A. Angles F and H are congruent.B. Angles A and G are congruent.C. Angles A and H are congruent.D. Angles E and C are congruent. 15x + 10 - 6 PLEASE HELP ME SOLVE THIS PLEASE SHOW WORK TO? THANK YOU 70 feet in 2 rolls as a unit rate if f(x)=10x+3 whar is the value when f(x)=19 The text compares the life people live today with the life people lived during the Middle Ages. According to the text, what is one difference between life today and life during the Middle Ages? Life likely had a faster pace during the Middle Ages than it does now. Life likely was more fun during the Middle Ages than it is now. Life likely had a slower, steadier pace during the Middle Ages than it does now. What was one important feature of the Akkadian Empire in Mesopotamia?A.It was the world's first empire.B.It developed the wheel and axle.C.It gave equal rights to men and women.D.It created the earliest writing system. What is the difference between a Primary Election and a Caucus? F (x) = x^2 + 4x; Find f (7) The giant african land snail is an invasive species that is also the largest snail ons earth what is most likely consequence of introducing this invasive species into a stable ecosystem?A. The native snail species will change their diet therefore destabilizing the ecosystem.B. The native snail species will move to another area allowing the ecosystem to remain stable.C. The native snail species will share their food with the invasive species stabilizing the ecosystem.D. The native snail species will compete for resources with the invasive species and destabilize the ecosystem what is the meaning of life why are we here why does anything matter What is this in english "On peut aller au nouveau centre commercial de lautre ct de la rue?" Cardiac muscles are considered smooth musclesTrue or False how to find a inequality An earthworm times 2 cm into the ground each day the earth worm tunnels at about the same place every day how many days will it take the earthworm to tunnel 14 cm