what is the correct order of steps in a gram staining procedure? 1. iodine is added as a fixative or mordant. 2. the specimen is treated with a stain such as safranin or fuchsin (a pink stain). 3. the specimen is flushed with alcohol to dehydrate peptidoglycans and trap the stain. 4. the specimen is treated with a stain called crystal violet.

Answers

Answer 1

The correct order of steps in a gram staining procedure is:

(4) the specimen is treated with a stain called crystal violet.

(1) iodine is added as a fixative or mordant.

(3) the specimen is flushed with alcohol to dehydrate peptidoglycans and trap the stain.

(2) the specimen is treated with a stain such as safranin or fuchsin (a pink stain)

Gram staining is a common staining technique which helps to classify the bacteria into two categories: Gram positive and Gram negative. Gram positive bacteria appear violet in color and Gram negative bacteria appear to be pink. This color difference appears due to the difference in the bacterial cell walls.

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Related Questions

Sammy is doing research that involves placing objects in the visual fields of visually impaired elderly men. you would suggest that sammy place the electrodes near the _______________

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In Occipital lobe part of the brain can sammy place the electrodes.

One of the four main lobes of the cerebral cortex of the mammalian brain is the occipital lobe. The term comes from the Latin words ob, meaning "behind," and caput, meaning "the head," and refers to its location at the back of the head.

The majority of the physical region of the visual cortex is located in the occipital lobe, which is the mammalian brain's visual processing hub. The primary visual cortex is designated as Brodmann area 17, or V1 (visual one). Human V1 is situated in the calcarine sulcus, on the medial side of the occipital lobe.

The Occipital Lobe of the brain is the area that enables us to comprehend and perceive with accuracy what the eyes are perceiving and seeing. Processing the pictures that the eyes are seeing is another job of the occipital lobe. The occipital lobe performs more than just visualizing images received from the eyes; it also recognizes colors, which is necessary for reading and comprehension, as well as the movement of objects.

Correct Question:

Sammy is doing research that involves placing objects in the visual fields of visually impaired elderly men. He wants to discover what specific neurons fire in response to the features of the objects. The study requires electrodes to be implanted in the men's brains. In which part of the brain would you suggest Sammy place the electrodes?

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In a classic medical case study, it was written that phineas gage became emotionally labile and unable to restrain his impulsive behaviors after a severe head injury. While this account appears to have been vastly overstated, because of this story, these functions were associated with damage to which part of the brain?.

Answers

The functions are associated with damage to the frontal lobes part of the brain.

The frontal lobe is a part of the brain that controls important processes such as consciousness and communication, memory, attention, and others. In the brain, the frontal lobe is a part of the cerebral cortex. Injury to the prefrontal cortex in the frontal lobes of the brain can create dramatic personality changes in the absence of any obvious neurological impairments, as in Gage's case.

The prefrontal cortex's cardinal role is temporal organization of behavior, which is supported by the subordinate functions of short-term memory, motor attention, and inhibitory control. Gage's personality shift would be compatible with injury to the orbitofrontal cortex of his frontal lobe's ventral aspect, influencing his emotions.

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In Sl units, the standard unit of force

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In Sl units, the standard unit of force newton, symbol N.
The base units relevant to force are:

The metre, or m, is a unit of length.

The kilogram (kg), a unit of mass

second, or s, is a unit of time.

The change in momentum rate is referred to as force. This is comparable to mass x acceleration for a constant mass.

Therefore, 1 N equals 1 kilogram m s-2 or 1 kg m/s2.

Force is denoted by the letter F.

A force is an effect that can alter an object's motion in physics. A force can cause an object with mass to accelerate when it changes its velocity, for as when it moves away from rest. An obvious way to describe force is as a push or a pull. A force is a vector quantity since it has both magnitude and direction. It is calculated using the newton SI unit (N). Force is denoted by the letter F.

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answer this please!!!!!!!!!

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DNA is transcribed into RNA, then RNA is translated into proteins

you would like to see if the gene that you are studying in mouse has a homolog in humans. to do this you decide to do a southern blot. you believe the gene sequence will be not be highly conserved. therefore, you would like to select conditions to hybridize your probe with low stringency to find as many candidates as possible. which condition (relatively) will you use? select one: a. at higher annealing temperature b. at lower annealing temperature

Answers

So option a(High stringency) is the correct answer. You will employ (relatively) high stringency.

The gene sequence will be not be highly conserved. As a result, you want to choose criteria to hybridise your probe with minimal stringency in order to find as many candidates as feasible.

What exactly is a gene?

A gene is the most basic physical and functional component of heredity. The building block of genes is DNA. Some genes act as blueprints for protein synthesis. However, many genes do not encode proteins. Human genes range in size from a few hundred to over 2 million DNA bases.

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.The protective outer shells of coral animals are formed from

Answers

Answer: limestone

or a calcium carbonate

Explanation:

1. . What is facilitated diffusion?


2. Name one type of proteins are involved in facilitated diffusion?


3. True / False: Diffusion of water and facilitated diffusion require energy from a cell.

4. Diffusion of water and facilitated diffusion are forms of
transport.
__________________



5. What is osmosis?


6. Controlling osmosis is important in helping a cell maintain
which is a stable internal environment.
_____________________


7. In osmosis, water molecules move toward the region of
concentration.
___________________


8. Osmosis works because water molecules (solvent) can move through a membrane, but __________________________ cannot.

Numbers 9 and 10 are on the next page.

9. Two solutions that have equal concentrations are said to be ________________.

10. If a red blood cell is placed into a hypertonic solution, what will happen to that red blood cell?

Answers

Facilitated diffusion is a type of passive transport of molecules across the plasma membrane.

What is facilitated diffusion?

1. Facilitated diffusion is a passive transport of molecules, which means that it does not spend energy to be able to generate the passage through the membrane. So the molecule is going to be passing down its concentration gradient.

2. One class of proteins involved in facilitated diffusion is carrier proteins.

3. False: Diffusion of water and facilitated diffusion does not require energy expenditure.

4. Diffusion of water and facilitated diffusion are forms of passive transport.

5. Osmosis is a process in which there is a movement of water across a semi-permeable membrane.

6. Controlling osmosis is important in helping a cell maintain which is a stable internal environment it's true.

7. In osmosis, water molecules move toward the region of higher concentration of molecules.

8. Osmosis works because water molecules (solvent) can move through a membrane, but solutes cannot.

9. Two solutions that have equal concentrations are said to be isoosmotic.

10. If a red blood cell is placed into a hypertonic solution, the cell will shrink as the water will leave it in order to equalize the concentrations.

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what type of medication is important to a person having pneumonia that doctor recommend? ​

Answers

Answer:

He should take Antibiotics

Explanation:

Eukaryotic cells store their dna in the form of linear chromosomes. This creates problems for replication of dna at the ends of the chromosomes because.

Answers

Answer:

DNA polymerase can only synthesize DNA in the 5' to 3' direction.

Explanation:

I did this already. "If it is a written answer, make sure you change wording!"

please click thanks & rate! also, brainliest ?

Predict what happens to cell membrane composition in the brain of a wolf living in yellowstone national park with hot summers and cool winters.

Answers

Okay so I believe they adapt to the environment

halldorsson s, gudjonsson t, gottfredsson m, singh pk, gudmundsson gh, and baldursson o (2010) azithromycin maintains airway epithelial integrity during pseudomonas aeruginosa infection. am j respir cell mol biol 42:62–68.

Answers

Study on use of ivermectin for Covid-19: Azithromycin maintains airway epithelial integrity during Pseudomonas aeruginosa infection.

Overview:

The macrolide antibiotic azithromycin (azm) has been shown to improve lung function in CF patients without reducing bacterial counts in the lungs.

Tight junctions (TJs) play an important role in maintaining the integrity of the bronchial epithelium. Patients with chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF) often suffer chronic infections with the opportunistic Gram-negative bacterium Pseudomonas aeruginosa, which produces several virulence factors, including rhamnolipids.

However, the mechanism of this effect is still debated. Azm was previously shown to increase transepithelial electrical resistance (TER) in bronchial epithelial cell lines.

In this study, we used an air-liquid interface model of human airway epithelium to measure changes in TER, TJ expression and structure after exposure to live Pseudomonas aeruginosa PAO1 and PAO1-Deltarhl, PAO1 mutants lacking rhlA and rhlB. Did. Enzyme for rhamnolipid production.

Additionally, cells were challenged with bacterial culture media conditioned by these strains, purified rhamnolipids, or synthetic 3O-C(12)-HSL. A virulence factor secreted by Pseudomonas aeruginosa reduced TER in the bronchial epithelium, causing TJ rearrangements and exposing the epithelium to further bacterial invasion. Pretreatment of bronchial epithelium with Azm attenuated this effect and promoted epithelial repair.

These data suggest that azm protects the bronchial epithelium during Pseudomonas aeruginosa infection independently of its antibacterial activity, partially explaining the beneficial results observed in clinical trials in patients with CF.

complete question, Study on use of ivermectin for Covid-19: Azithromycin.

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the adult human brain is composed of billions of neurons and glial cells connected to one another. what are some of the challenges of succesfully grafting donor neural cells to treat brain disease?

Answers

Answer:

One challenge is finding cells that will survive and function in the recipient's brain. Another challenge is that the grafted cells must be able to integrate into the existing neural circuitry and establish appropriate connections. Additionally, the grafted cells must be able to withstand the immunological response of the host brain. Finally, the grafted cells must be able to function over the long-term in order to be clinically effective.

So how can this be solved? (Bonus answer!)

Donor cells can be grafted to treat brain diseases by replacing damaged or destroyed cells with healthy cells. This can be done with stem cells, which are cells that can develop into many different types of cells, or with specific types of cells that are needed to treat a particular disease.

What are neurons?

Neurons are cells in the nervous system that transmit information to other cells in the body. There are three types of neurons: sensory neurons, motor neurons, and interneurons.

Sensory neurons are neurons that detect stimuli from the environment and send signals to the brain.Motor neurons are neurons that send signals from the brain to the muscles, causing them to contract.Interneurons are neurons that transmit signals between other neurons.

What are Glial cells?

Glial cells are cells in the nervous system that provide support and protection for neurons. There are three types of glial cells: astrocytes, oligodendrocytes, and microglia.

Astrocytes are the most numerous type of glial cell and are responsible for providing nutrients and support to neurons.Oligodendrocytes are cells that wrap aroundaxons and provide myelin, a fatty substance that insulates axons and helps them to conduct electrical signals.Microglia are cells that protect the brain and spinal cord from infection and damage.

Which test would measure the coating of red cells by antibody as occurs in hemolytic transfusion reactions? *

Answers

Answer:

DAT(Direct Antiglobulin Test)

The genome of escherichia coli consists of 4380 genes, encoding 4290 proteins, in just 5175720 base pairs. what percent fraction of the escherichia coli genes encode proteins?

Answers

According to the above formula, the percentage of genes that code for proteins is 97.95%.

Briefing:

4380 genes are found in total.

4290 total proteins are encoded by the genes.

There are 5175720 base pairs overall.

Percentage of genes that code for proteins: Although there are 4380 genes overall, only 4290 of them code for proteins.

Total number of protein-coding genes divided by the total number of genes present gives the fraction of protein-coding genes (4290/4380), which equals 0.9795.

Percent fraction of genes encoding proteins

= Fraction of genes encoding proteins * 100

= 0.9795*100

The percentage of genes that code for proteins is 97.95%.

Where are the genes in DNA?

Deoxyribonucleic acid (DNA) segments called genes are responsible for carrying out specified protein functions in one or more types of bodily cells. The structures called chromosomes found in cells house a person's genes. Chromosomes, which are found in the cell nucleus, are where genes are found.

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the ganglion that supplies postganglionic parasympathetic innervation to the submandibular and sublingual salivary glands to increase their secretions is the

Answers

-The Submandibular Ganglion is the ganglion that provides postganglionic parasympathetic innervation to the submandibular and sublingual salivary glands to augment their secretions.

-The human autonomic nervous system includes the submandibular ganglion, often known as the submaxillary ganglion in earlier sources. It belongs to the head and neck's four parasympathetic ganglia. (The otic ganglion, pterygopalatine ganglion, and ciliary ganglion are the others.)

Where is it found?

Little and fusiform in shape, the submandibular ganglion is modest. It is located on the hyoglossus muscle, close to the posterior border of the mylohyoid muscle, over the deep part of the submandibular gland.

The ganglion "hangs" from the lower border of the lingual nerve by two nerve filaments (itself a branch of the mandibular nerve, CN V3). Two anterior and two posterior filaments support it from the lingual nerve. It receives a branch from the chorda tympani nerve, which runs in the sheath of the lingual nerve, through the posterior of these.

Fibers:

The submandibular ganglion, like other parasympathetic ganglia of the head and neck, serves as the synapse for parasympathetic nerve fibres as well as carrying other types of nerve fibre that do not synapse in the ganglion. The following is a list of the fibres carried in the ganglion:

sympathetic fibres from the face nerve and its branches, which travel through the external carotid plexus. In this ganglion, they do not synaptize.

Preganglionic parasympathetic fibres from the superior salivary nucleus of the Pons travel to this ganglion through the chorda tympani and lingual nerve.

salivary glands of the submandibular, sublingual, and oral mucosa are all served by postganglionic parasympathetic fibres. They act as these glands' secretomotors. After re-entering the lingual nerve, some postganglionic fibres travel to the sublingual gland.

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Which part of the phospholipid bilayer is hydrophobic? Which direction does it face?

Answers

The fatty acid tails is hydrophobic
They face inward

What is the mantle composed of? *
O Magma
Lava
O Liquid metal
O Solid rock
H

Answers

The earths mantle is made of solid rock, brainliest pls.

What macromolecules make up membranes?
A. proteins
B. carbohydrates
C. nucleic acids
D. lipids

Answers

Answer:

Proteins.

Explanation:

The key ingredients of a cell membrane are phospholipids, proteins, and carbohydrate groups which help to form the cell membrane.

It’s a proteins
I and did the same thing

How does eye location best represent the relationship between structure and function?

The location of the eyes are how they select favorable mates.

Predator eye location on the front of the head allows them to see prey hiding off to the side .

The eyes that are located in the front can see better at night .

Prey eye location on sides of the head allow for greater field of view.

Answers

Answer: Prey eye location on sides of the head allow for greater field of view.

Explanation: I am like 99% sure that is correct if not pls let me know I remember doing this exact paper

what’s the answer? i need it for my biology homework.

Answers

Answer: (C) The sodium-potassium pump, which restores resting membrane potentials in neurons through the use of ATP

What are some of the problems with our continuing reliance on fossil fuels?

Answers

Answer:

it will cause health problems, destroy our wild places, and release toxins such as mercury and arsenic into our communities.

Answer:

Fossil fuels pollute the environment

Fossil fuels are non-renewable and unsustainable

Drilling for fossil fuels is a dangerous process

Explanation:

if you want to determine the proportion of cells that have been killed by a particular antimicrobial treatment, it would be best to do which type of cell count before and after that treatment?

Answers

Agar plates are the standard solid support material for growing microorganisms.

What is Agar plates?

Agar plates are Petri dishes used to cultivate microorganisms that contain a growth media that has been hardened with agar. Antibiotics are one example of the selective chemicals that are occasionally added to affect growth.

96 pinner for use in spot experiments with yeast, fungal, or bacterial cells

Individual microorganisms placed on the plate will develop into distinct colonies, each of which is a clone genetically identical to the distinct ancestor organism (except for the low, unavoidable rate of mutation). As a result, the plate can be used to estimate the number of organisms in a liquid culture or a suitable dilution of that culture using a colony counter, as well as to create genetically pure cultures from a mixed culture of genetically diverse organisms.

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PLEASE HELP ME WITH THIS​

Answers

Answer:circle pentagon rectangle

Explanation:

Which part of the scientific method might happen when an idea from biology is combined with an idea from engineering?

Answers

Perhaps the part of the scientific method that involves biology combining with engineering can be the last part. The last part being when the experimental results are shared with other scientists to compare results and examinations.

Formulation of a Hypothes is a part of the scientific method might happen when an idea from biology is combined with an idea from engineering.

When an idea from biology is combined with an idea from engineering, the formulation of a hypothesis is likely to occur. This involves generating a testable statement or prediction that can be investigated through experimentation and testing.

In this case, the integration of biological and engineering concepts could lead to the development of a hypothesis that aims to solve a specific problem or address a question by utilizing both fields' principles and knowledge.

The given question is incomplete, the complete question is

Which part of the scientific method might happen when an idea from biology is combined with an idea from engineering?

A. Observation and Data Collection

B. Formulation of a Hypothesis

C. Experimentation and Testing

D. Analysis and Conclusion

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Wild-type mice have brown fur. You have two strains of pure breeding mutant mice, both of which have white fur. When you cross the two strains with each other, all of the progeny have wild-type brown fur. What does this genetic complementation tell you?.

Answers

The phenotypic of coat color is influenced by several genes.

A gene have 2 alleles which decide the phenotype character. By crossing 2 mutant strain can result in the formation of wild type mice which have brown fur.

Gene- In every type of living creature, a gene is a highly precise sequence of nucleotide monomers that has the power to fully or partially regulate the expression of one or more features. Deoxyribonucleic acid (DNA) and, in the case of certain viruses, ribonucleic acid (RNA) polymers are used to create genes.

Phenotype- An observable characteristic is simply referred to as a "phenotype." Pheno, which has the same origin as the term "phenomenon," simply means "observe." It may therefore relate to anything ranging from a common attribute, like height or hair color, to the absence or presence of a disease. It is also an observable kind of an organism.

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What monomers do shapes B, C, D and E represent?
Which macromolecule did you build?

Answers

The macromolecule which is formed or build is lipid.

What is lipid?

Lipid has been described  as an organic compound which has been not  able to dissolve in the water. The example of the lipid are oils, waxes, hormones, as well as fats. The main function or performance of the lipid has to maintain the structure or the components of the cell membranes, that storage site of energy, and important molecule of the cell signaling.

A monomer has been defined as a type of molecule that is able to  make chemical bond with any of the molecule and has been  form a long chain and polymer in a sequence of the chain of an unspecified or unknown number of the monomers.

Therefore, the macromolecule which is formed or build is lipid.

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4. Calculate the actual size of one cell in each of the following microscope Images, as seen on low
power. Assume the FOV is 5.0 mm.

Answers

To calculate the size of the magnified cell we can use the following formula

Actual size = Image size(with ruler) / Magnification.

How does a microscope function to determine the size of a cell?

Divide the number of cells that span the field of view's diameter by the field of view's diameter to determine a cell's length. If 50 cells are aligned end to end to form a field with a diameter of 5 mm, then 5 mm/50 cells equals 0.1 mm/cell.

What is the equation for magnification image size and actual size?

Actual size = image size / magnification.

Image size = magnification x actual size.

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Biology and geology are two very different sciences, but biologists and geologists use scientific knowledge in the same way. These scientists both use scientific knowledge to
answer questions and solve problems affecting the natural world.
investigate the chemical composition of the Earth’s different layers.
observe and classify living organisms into groups.
explain the rituals and traditions of human cultures.

Answers

Biologists and Geologists use scientific knowledge to answer questions and solve problems affecting the natural world.

Biologists specialize in the study of living things. Geologists are considered physical scientists since they study the earth. Geology and biology are generally unconnected areas, despite the fact that they are both natural sciences, or studies that investigate the physical universe.

Scientific knowledge is what Biologists and Geologists gain from the experimental and data-gathering phases of the scientific method. Data gathering for scientific research is done in order to study and understand a phenomenon with help of which questions are answered and problems are solved.

While biology is the study of living things and rocks are not live things, geology is the study of rocks. A biologist, however, may find geologist information beneficial, and vice versa.

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You are doing a study on the enzymatic effects of lysosomes and want to follow the whole process from the formation of the enzymes to their use in the lysosome. In what location of the cell would you begin this observation?.

Answers

You are doing a study on the enzymatic effects of lysosomes and want to follow the whole process from the formation of the enzymes to their use in the lysosome. Endoplasmic reticulum (ER) is the place where this observation will begin.

The endoplasmic reticulum (ER) is the site of the body which is involved in protein synthesis. The Rough endoplasmic reticulum (RER) has ribosomes attached to it. The ribosomes are the sites where the process of translation occurs. The Endoplasmic reticulum is known to play a central role in not only the formation of proteins but also the formation of lipids.

Hence, if we want to study lysosomes from the beginning i,e from the formation of enzymes present in lysosomes, then we will start from the Endoplasmic reticulum as it is the membrane is the place where lysosomes along with various other proteins are formed.

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the zebra longwing butterfly (heliconius charitonius) has adapted to withstand the toxins and defenses in its host plant, the passion flower (passiflora sp.). this back-and-forth response of adaptations between the butterfly and plant that has lasted thousands of years is .

Answers

This back-and-forth response of adaptations between the plant and butterfly that has lasted thousands of years is Coevolution.

What is coevolution?

Coevolution occurs when the genetic development of two or more species has an impact on one another's evolutionary processes. Hummingbirds and ornithophilous (bird-pollinated) flowers are two examples. The nectar from the ornithophilous blooms, which has a high sugar content, provides food for the birds. In exchange, the birds help pollinate these flowers.

As mentioned in question, the zebra longwing butterfly (Heliconius Charitonius) has adapted to withstand the toxins and defenses in its host plant the passion flower (Passiflora sp.). This back-and-forth response of adaptations between the plant and butterfly that has lasted thousands of years is coevolution.

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Review the image. What impact did Dorothea Dix have during the Antebellum reform movement?Group of answer choicesHer efforts increased awareness regarding the discrimination of immigrants.Her efforts caused the privatization of elementary and secondary schools.Her efforts improved the conditions of prisons and asylums.Her efforts established prohibition laws on alcohol in many states. find the product of two numbers whose sum is 11 and diffrence is 3 The following are some changes that may take place in the market for textbooks. For each of the following, indicate what will happen to either the demand for or the supply of textbooks by listing which curve is affected and then the terms: "shift right or "shift left". if 4n=3.60 what is the value of n What is the equation of this graphed line? Why did the Israelites have to fight when they returned in Canaan 59.) ""a lease that provides for specified changes in the amount of rent at one or more points during the lease term"" is called a(n) lease. A scientist discovers that there is a virus that turns pilbugs purple when they become infected, the virus alsoslowty kills them. She wonders if this vrus is spreadthrough ar or through contact. She sets up four smalchambers for her experiment, each has its own isolatedor supply. Each has 5 purple pill bugs and 30 gray normal pl buns, all thesame age, size, and sex One chamber has a divider separating the purplpill bugs on the left and the normal pl bugs on the right. The dvderallows air to flow from the left side to the right side, but pl bugscannot pass through the divider: The second chamber has a diderseparating the infected and noninfected plibugs, but this divider does nolet ar pass through it, each side of the chamber has its own ar supply.A third chamber is set up with no dividen, the pill bugs can interact witheach other. In the Fourth chamber, she places only 50 noninfected plbugs with no divider. Each day over a month, she observes the pl bugsand records how many turn purple and how many die.what is the independent variable, dependent variable, constants, control group, and experimental group? 35 x dash x 37 = 10,360 what can be said about the number in the box Find the value of y if M is the midpoint of LN. LM=9y-4 MN=6y+5 LN? 2. Evaluate the role that the federal government played in one of the following three LaborDisputes: Homestead Strike, Pullman Strike or Lawrence Textile Strike. Write each expression in simplest form. (-27x)/ What was the mass of the ethanol plus the graduated cylinder when 20.0 ml of ethanol was added? choose the closest answer. a sporting event coordinator has 200 mini foam fingers. She gives 2 foam fingers to each student in attendance. there are 26 left. how many students are in attendance What is the relationship between the standard deviation and the accuracy of a procedure?. Simplify the expression.-1- 2/3(6/7-3/7n) question chitinase is an enzyme that breaks down the chitin molecules in the insect exoskeleton. the sequence of amino acids in the chitinase is an example of which level of protein structure? secondary secondary, tertiary tertiary primary primary quaternary quaternary 6 in.4 in.6 in. Math do these ratios form a proportion? 5:10 11:14 The chart probably contains more data from volcanoes over the past 300 years because? Help!