You start with 100 units of protein activity and 100 grams of total protein. After the first centrifugation step, you have 15% less activity and 80% less total protein. What is your fold-purification of the target protein?.

Answers

Answer 1

Fold-purification of the target protein  is  mathematically given as

Fold= 4.25

This is further explained below.

What is your fold-purification of the target protein?.

Generally, the equation for Specific activity is  mathematically given as

x= Activity (units)/Protein (mg)

Therefore

X= 100/100000

X= 0.001

After centrifugation

X'= 85/20000

X'= 0.00425

Generally, the equation for Fold  is  mathematically given as

[tex]Fold= \frac{x'}{x}[/tex]

Fold= 0.00425/0.001

Fold= 4.25

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Related Questions

The process in which nitrogen is converted into a usable form for life is called _______. a. nitrogen fixation b. photosynthesis c. greenhouse effect d. photolysis

Answers

Correct option a) Nitrogen fixation

The process in which nitrogen is converted into a usable form for life is called nitrogen fixation.The conversion of nitrogen fueloline ( N 2) into nitrates and nitrites via atmospheric, business and organic strategies is known as nitrogen fixation. Atmospheric nitrogen need to be processed, or "fixed", right into a usable shape to be taken up with the aid of using plants.

Nitrogen fixation, any herbal or business system that reasons loose nitrogen (N2), that's a fantastically inert fueloline ample in air, to mix chemically with different factors to shape more-reactive nitrogen compounds including ammonia, nitrates, or nitrites.

For nitrogen to be to be had to make proteins, DNA, and different biologically critical compounds, it need to first be transformed right into a exceptional chemical shape. The system of changing N2 into biologically to be had nitrogen is known as nitrogen fixation.

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I am a grass and a cereal grain. many food products are made with me. Some might have allergies to me. what plant am i?

Answers

Answer:

The answer is wheat.

Pick a type of cell. Muscle, Nerve, Brain, Cardiac, Digestive, etc. Include Picture and unique properties of that cell. What organelles does it contain? What organelles does it not have.

Answers

Answer: All cells have membranes (the building), DNA (the various blueprints), and ribosomes (the production line), and so are able to make proteins

Explanation:

Why is nitrogen scarce in the biosphere?

Answers

Answer: nitrogen gas cannot cycle out of the atmosphere and into organisms

Explanation:

Answer:

The Earth Is Facing a Nitrogen Shortage Due to Climate Change, Study Says. Greenhouse gases, among many other pressures, are reducing access to this key nutrient in ecosystems around the world.

Explanation:

Although nitrogen is very abundant in the atmosphere as dinitrogen gas (N2), it is largely inaccessible in this form to most organisms, making nitrogen a scarce resource and often limiting primary productivity in many ecosystems.

True or false. only glucose can be used to produce atp via cellular respiration in animal cells.

Answers

Only glucose can be used to produce atp via cellular respiration in animal cells is false.

The primary sugar in your blood is called blood sugar, or glucose. Your body uses it as its primary source of energy, and it originates from the food you eat. All of the cells in your body receive glucose from your blood to be used as fuelIon transport, muscular contraction, nerve impulse transmission, substrate phosphorylation, and chemical synthesis are just a few of the processes that use ATP as an energy source. There is a significant need for ATP in both of these processes and others. Being a complicated organism, the body needs energy to continue operating properly.  Due to the phosphate groups that connect via phosphodiester bonds, ATP is a great energy storage molecule to employ as "money."

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1. What would happen if a population were
to exceed its carrying capacity?
a. More organisms of that species
will be born.
b. The death rate of the population
will rise.
c. The predators of this population
will decrease.
d.
Immigration of more organisms of
that species will take place.

Answers

If a population were to exceed its carrying capacity The death rate of the population will rise.

The correct option is B.

What is death rate?

A mortality rate is a measurement of how frequently people die within a given population during a certain period of time. The choice of measuring disease or death depends on whether you want to use morbidity or mortality metrics, which are frequently mathematically equivalent.

How is the death rate calculated?

A crude date rate is calculated by dividing the total number of deaths among residents of a given geographic area (a nation, state, county, etc.) by the total population for the same geographic area (for a given time period, typically a calendar year), then multiplying that result by 100,000.

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Select the statements about meiosis that are true.

Answers

Select the statements about meiosis that are true.

The daughter cells of meiosis have half as many chromosomes as the parent cell.Meiosis produces gametes.Meiosis produces four haploid cells.Meiosis produces nonidentical cells.

The statements are all true

What is meiosis?

The term meiosis is used in biology to mean the cell division peculiar to organisms which are involved in sexual reproduction, it helps in decreasing the number of chromosomes in the egg and sperm. For human beings, the have two sets of chromosomes. During the process of fertilizations each of the parent donates a chromosome which merge to form one

The two sets of chromosomes is referred to as diploids, one from each parent. Two rounds of division of the diploid cell gives four daughter cells called haploid. The egg and sperm are referred to as gametes and are haploids. The haploid cells produced are nonidentical

As regards the question, the information read will help to conclude that all the options are true.

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complete question

Which of the following statements is/are true about meiosis? Select all that apply.

-The daughter cells of meiosis have half as many chromosomes as the parent cell.

-Meiosis produces gametes.

-Meiosis produces four haploid cells.

-Meiosis produces nonidentical cells.

Semipermeable cell membranes are made of phospholipids that form a bilayer. What major benefit does this arrangement of the membrane provide to the cell?.

Answers

Answer:it only allows certain molecules to pass through it

Explanation:

semipermeable membrane is a type of biological synthetic, polymeric membrane that will allow certain molecules or ions to pass through it by osmosis

When purified cytochrome oxidase is exposed to cyanide, the cyanide binds reversibly and with high affinity to the enzyme’s active site. What will be the likely outcome if mitochondria are exposed to high levels of cyanide?.

Answers

The likely outcome, if mitochondria are exposed to high levels of cyanide, will be that the cyanide will prevent the movement of electrons along the electron transport chain. The correct answer is option(c).

Cyanide inhibits cytochrome C oxidase, an essential component of the electron transport chain and a common name for Complex IV. By obstructing this complex, cyanide basically prevents the flow of electrons across the chain. As a result, no proton gradient can be produced since protons cannot be forced out of the matrix and into the intermembranous space. In the fourth protein complex, oxygen serves as the ultimate acceptor for the electrons.

The electrochemical gradient is then further strengthened when the oxygen with its extra electrons combines two hydrogen ions to form water. Without oxygen, the mitochondria would not be able to take electrons from the system, and ATP synthase would not produce ATP in the absence of a proton gradient and a flow of protons through it.

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The complete question is:

''When purified cytochrome oxidase is exposed to cyanide, the cyanide binds reversibly and with high affinity to the enzyme's active site. What will be the likely outcome if mitochondria are exposed to high levels of cyanide?

A. The cyanide will prevent the movement of electrons along the electron transport chain.

B. The cyanide will block the movement of protons across the inner mitochondrial membrane.

C. The cyanide will inhibit the transfer of electrons from NADH to initiate electron transport.

D. The cyanide will prevent oxygen from reaching the mitochondrial matrix.

which laundry detergent removes stains more effectively?

What is the independent and dependent variable?

Answers

Enzyme-containing laundry detergents will remove stains more effectively than detergents without enzymes. Stains will be removed more successfully from clothing with homemade laundry detergent than from commercial detergents.

A variable that is independent is precisely what it sounds like. It is a stand-alone variable that is unaffected by the other variables you are attempting to assess. Age, for instance, could be an independent variable. A person's age won't alter as a result of other circumstances like what they eat, how much they attend school, or how much television they watch. In actuality, when attempting to establish a link between two variables, you are attempting to determine whether the independent variable affects the dependent variables in any way.

A dependent variable is precisely what it sounds like, just like an independent variable. It is something on which other elements depend. A test score, for instance, maybe a dependent variable because it could vary based on a number of variables, including how much you studied, how much sleep you got the night before the test, and even how hungry you were.

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help me with this pls i will mark as brainliest​

Answers

Explanation:

once you get more than 1 answer of a question. A message is shown above each answer that is "Mark as brainiest". Click on the option to mark it the best answer ..1

According to the linnaean system of classification,mushrooms, mold, and mildew all belong to the kingdom fungi.why are these organisms given the same classification

Answers

According to the Linnaean system of classification, mushrooms, mold, and mildew all belong to the kingdom fungi because: -

The absence of chlorophyll is what distinguishes fungus from plants.Fungi belongs to Eukaryotic group. Some fungus are unicellular, but the majority are multicellular.Fungi do not require sunshine to survive.

Linnaean system of classification- Because it encouraged the application of binomial nomenclature to distinguish between each species, the Linnaean system is significant. Researchers may interact without relying on deceptive common names after the approach had been accepted. No regardless of the language a human spoke, they were still considered a member of the Homo sapiens species.

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How does energy from the sun fuel life on earth?

Answers

Answer:

We need vitamin D which is given by the sun. Vitamin D helps keep our skin healthy, and plants also need these vitamins to function and live their lives on a day-to-day basis. Without the sun, the earth would be a very cold rock, and everything would slowly die off.

Answer:

The sun provides us with light and solar radiation which basically makes it possible for life to exist on Earth in the first place. Trees provide us with oxygen, which we as human beings use our lungs and respiratory system to breathe. In order for there to be trees and plants they depend on the sun which allows them to grow and create food for themselves. Not just humans, but animals as well does the sun provide for. Sunlight, the energy from the sun, sustains the process of life.

Explanation:

The cell membrane of an animal cell was damaged, preventing the cell from performing one of its functions. Which cell function would be lost, and how would this loss affect the cell?.

Answers

The cell membrane of an animal cell was damaged, preventing the cell from performing one of its functions. The function controlling the exit and entry of material from the cell would be lost and the cell would eventually die.

The cell membrane works as a screen to keep the cell separate from the outer system. Its function is to only allow the required substance into the cell. It is made up of lipids and proteins.

If it is damaged then every substance and molecule will start to enter or leave the cell. There would be no selection which would result in the loss of all the functional properties of the cell.

When there is a damage to the parts of the cell and it fails to perform the necessary functions, it will die eventually.

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the organelles are like because ?

Answers

An organelle is a subcellular structure that has one or more specific jobs to perform in the cell, much like an organ does in the body

The diagram above best illustrates major processes in which of the following biochemical cycles?
Carbon cycle
Nitrogen cycle
Phosphorus cycle
Water cycle

Answers

I believe this is the Phosphorus cycle.

The diagram above best illustrates major processes of the following biochemical cycles is phosphorus cycle. Hence option 3 is correct.

What is biochemical cycle?

Biochemical cycle is defined as a naturally occurring cycle that travels through biotic and abiotic environmental components. A biogeochemical cycle's primary function is to recycle the elements on earth. The biogeochemical cycle makes it possible for matter to change its form. It includes biotic components like rocks, air, water, and chemicals as well as zoological factors like live things.

Phosphorus cycle is defined as a cycle in which phosphorus is transported across the lithosphere, hydrosphere, and biosphere throughout a biogeochemical process. The phosphorus cycle is significant because phosphorus is a crucial ingredient for supporting life on Earth. It also plays a crucial role in the structure of genetic material, the function of cell membranes, and the development of bones and teeth.

Thus, the diagram above best illustrates major processes of the following biochemical cycles is phosphorus cycle. Hence option 3 is correct.

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4. Which describes the effects of population increase
and resource depletion?
A. increased competition
B. increased emigration
C. exponential population growth
D. straight-line population growth

Answers

Answer:

a

Explanation:

due to more organisms entering the environment they'll be less resources and more competition

Enzymes
Read the article and use the information to answer the following questions.

Enzymes

What two components are often found as part of an enzyme?

Answers

The two components that are often found as part of an enzyme are known as apoenzyme and cofactor or prosthetic group.

What are Enzymes?

Enzymes may be defined as biocatalysts that increase the rate of a chemical reaction without being consumed in the overall process. It may be thought that all cellular reactions and processes are mediated by enzymes.

Conjugated enzymes consist of two components. The non-protein component is known as the cofactor or prosthetic group. This component of protein is required for catalytic activity.

Elimination of this non-protein part from conjugated enzymes leaves only the proteinaceous part which is known as apoenzyme. This component of enzymes is biologically inactive.

Therefore, the two components that are often found as part of an enzyme are known as apoenzyme and cofactor or prosthetic group.

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What are The functions of the structure to the above are
O Support the body, store minerals, produce blood cells
O Moves the body and food through the intestines
O Send messages throughout body
O Protection, temperature regulation, Vitamin D production

Answers

Option D- Protection, Temperature regulation, Vitamin D production.

The given structure is of Skin- The epidermis, dermis, and subcutaneous layer are its three primary layers of the skin. The epidermis is an elastic outer layer that regenerates continuously. This is among its components: Keratinocytes, the primary epidermal cells produced at the base of the epidermis by cell division.

Some functions of skin are -

1. Protection - There is a thick layer of armor covering our entire body. The skin shields us from the majority of the outer world's hazards.

2. Sensation - We are able to feel sensation thanks to the thousands of tiny receptors found inside the layers of our skin.

3. Production of Vitamin D - A hormone, not a vitamin. Vitamin D production occurs on the skin. When in contact with sunshine, ultraviolet rays permeate the epidermis and convert provitamin D3 to pre vitamin D3.

4. Controls body's Temperature - The skin does an amazing job of stabilizing and regulating body temperature.

Hence, Option D is the most appropriate answer.

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Which organelles are ONLY found in plant cells?

Select all that apply.

Vacuole
Cell Wall
Chloroplasts
Nucleus
Endoplasmic Reticulum

Answers

Answer:

I think it's cell wall and chloroplasts

WILL GIVE BRAINLIREST

Which of the following explains the role of fruit bats as pictured in the rainforest food web shown above?




They are heterotrophs that depend on producers or other consumers for food.

They are saprotrophs that recycle organic waste into food.

They are autotrophs that use organic materials and sunlight to make food.

They are detritivores that rely on dead plant matter for food.

Answers

The sentence that explains the role of fruit bats as pictured in the rainforest food web shown above is they are heterotrophs that depend on producers or other consumers for food.

What do bats like to eat most?

Some bats are classified as nectarivores in terms of food, because their preferred menu is flower nectar and pollen. Most Brazilian bats prefer insects, while most around the world are frugivorous, that is, they feed on fruits.

Bats' diet varies by species. Thus, there are those who feed on fruits (frugivores), nectar and pollen from flowers (nectarivores), insects (insectivores), small vertebrates (carnivores) and blood (hematophages).

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I want to design an experiment to see if drinking coffee makes students
score higher on tests. What is the control group

Answers

1. The amount of coffee
2. What time is best to drink the coffee
3. Age group
4 . In what subjects
The students drinking coffee will be control group

The introductory passage discusses the importance of being able to study anme in a pure culture instead of in the consortium that exists in nature. Many bacteria exist in biofilms in nature. Based on the information in the passage and in the chapter, is it possible to create a pure culture of a species that normally exists in biofilms?.

Answers

Yes , because many species can have planktonic growth or live within a biofilm, and biofilm can contain a single species.

What exactly are biofilms and why are they crucial?

A biofilm is comparable to a well-fortified cave where microorganisms hibernate. Antibiotics are blocked by the matrix's barrier. But the entrance of food and oxygen is also restricted by this protective barrier. The bacteria in the biofilm's depths will begin to starve.

 There can be four effects of biofilm, generating chronic infection, the modulation of host immune response, antimicrobial drug resistance and contamination of medical devices.

So , yes it is possible.

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X inactivation occurs early in ______ embryonic development and prevents ______ of every gene on the x chromosome

Answers

X inactivation occurs early in female embryonic development and prevents a double dose of every gene on the X chromosome.

What is X inactivation?

The expression 'X inactivation' makes reference to an epigenetic phenomenon in which an X chromosome in female zygotes is inactivated during the first stages of development, which prevent double doses of genes that may be harmful to development during these first stages of development.

In conclusion, X inactivation occurs early in female embryonic development and prevents a double dose of every gene on the X chromosome.

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Which letter indicates the collagen fibers of the periosteum known as the perforating, or sharpey's, fibers?

Answers

The letter C indicates the collagen fibers of the periosteum which are known as the perforating or Sharpey's fibers.

Sharpey's fibers or the perforating fibers includes a matrix of connective tissue comprising of bundles of sturdy predominately Type I collagen fibers which connect periosteum to bone.

Periosteum refers to the membrane of blood vessels and nerves which cover most of the bones in the body. It provides blood supply to the bones and is also associated with the growth as well as repair of bones.

The question is incomplete. The complete question is:-

Use the diagram above to answer the following questions.

7) Which letter indicates the collagen fibers of the periosteum known as the perforating, or Sharpey's, fibers?

A) A

B) B

C) C

D) D

E) E

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Identify the macromolecule based on the structure.

Answers

Answer:

This macromolecule is carbohydrate

Proteins are synthesized at the ribosome and are often modified en route to their destination. How are these modified proteins most likely transported within a compartmentalized eukaryotic cell?.

Answers

Answer: Proteins are transported in vesicles between membrane-bound organelles.

Explanation: Proteins are 'packaged' into vesicles for transport within the cell. This prevents any unintended interactions from occurring.

The human population has changed in ways that are similar to other populations. How has the human population changed since it first appeared on earth?.

Answers

The population has generally increased at rates that have varied over time since it first appeared on earth,

This significantly altered after 1800: the world population was roughly 1 billion in 1800 and has expanded sevenfold since then. On our globe, around 108 billion humans have ever lived. This indicates that the current population accounts for 6.5% of all persons ever born.

The world population took hundreds of thousands of years to reach one billion, and then it increased sevenfold in just 200 years. In 2011, the world population surpassed 7 billion, and it is presently estimated to be about 7.6 billion. This rapid growth has been fueled mostly by an increase in the number of people of reproductive age, and has been accompanied by considerable changes in fertility rates, rising urbanization, and quicker migration. These trends will continue resulting in human population growth which are varied over time.

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Baby food manufacturers sometimes use proteases in their products. Proteases function by catalyzing the breakdown of protein, making digestion easier for babies. What type of molecule are proteases?.

Answers

If proteases function by catalyzing the breakdown of protein, making digestion easier for babies, then they are enzymes.

What are enzymes?

Enzymes are proteins that function to catalyze chemical reactions in biological systems by lowering the required activation energy to carry out these functions. These proteins (enzymes) contain an active site domain that binds to the particular substrate molecule (in this case protease enzymes bind to specific domains in proteins to catalyze breakdown).

In conclusion, if proteases function by catalyzing the breakdown of protein, making digestion easier for babies, then they are enzymes.

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which part of the digestive system first causes chemical changes to food

Answers

Answer:

Chemical digestion begins in your mouth. As you chew, your salivary glands release saliva into your mouth. The saliva contains digestive enzymes that start off the process of chemical digestion.

Explanation:

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